Real-time visualization of human prolactin alternate promoter usage in vivo using a double-transgenic rat model.

Molecular Endocrinology
Sabrina SempriniJohn J Mullins

Abstract

We have generated a humanized double-reporter transgenic rat for whole-body in vivo imaging of endocrine gene expression, using the human prolactin (PRL) gene locus as a physiologically important endocrine model system. The approach combines the advantages of bacterial artificial chromosome recombineering to report appropriate regulation of gene expression by distant elements, with double reporter activity for the study of highly dynamic promoter regulation in vivo and ex vivo. We show first that this rat transgenic model allows quantitative in vivo imaging of gene expression in the pituitary gland, allowing the study of pulsatile dynamic activity of the PRL promoter in normal endocrine cells in different physiological states. Using the dual reporters in combination, dramatic and unexpected changes in PRL expression were observed after inflammatory challenge. Expression of PRL was shown by RT-PCR to be driven by activation of the alternative upstream extrapituitary promoter and flow cytometry analysis pointed at diverse immune cells expressing the reporter gene. These studies demonstrate the effective use of this type of model for molecular physiology and illustrate the potential for providing novel insight into human gene expr...Continue Reading

References

Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E T RietschelF Di Padova
Oct 1, 1996·Genome Research·U E GibsonP M Williams
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S LiW T Garrard
Sep 12, 2000·Current Opinion in Genetics & Development·D Tautz
Apr 18, 2001·Transgenic Research·P Giraldo, L Montoliu
Oct 5, 2001·Nature Reviews. Genetics·N G CopelandD L Court
Jul 16, 2002·Annual Review of Biomedical Engineering·Christopher H Contag, Michael H Bachmann
Aug 17, 2002·Science·Michael B ElowitzPeter S Swain
Feb 22, 2003·Science·J Craig Venter
Jul 9, 2004·Reproductive Biology and Endocrinology : RB&E·Michael J Soares
Apr 2, 2005·Journal of Radiation Research·Junye LiuMasahiro Hiraoka
Jun 30, 2006·Developmental Biology·Caroline PopeAlan S McNeilly
Sep 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sarah GerloRon Kooijman
Dec 7, 2007·Endocrine Reviews·Nira Ben-JonathanElizabeth W LaPensee

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Citations

Feb 5, 2010·Journal of Cell Science·Claire V HarperMichael R H White
Oct 11, 2011·Journal of Cell Science·Karen FeatherstoneJulian R E Davis
May 3, 2011·PLoS Biology·Claire V HarperMichael R H White
Feb 2, 2016·ELife·Karen FeatherstoneJulian R E Davis
Dec 31, 2009·Clinical Endocrinology·Annice MukherjeeStephen Shalet
Mar 31, 2015·Biostatistics·Kirsty L HeyBärbel Finkenstädt
Nov 29, 2011·Current Opinion in Neurobiology·Carlos Lois, James O Groves
Mar 18, 2015·Nature Reviews. Endocrinology·Valérie BernardNadine Binart
Sep 18, 2012·Frontiers in Neuroendocrinology·P R Le TissierP Mollard
Oct 17, 2013·Journal of Molecular Endocrinology·Nelson D Horseman, Karen A Gregerson
Jun 11, 2010·Nature·David G SpillerMichael R H White
Dec 23, 2015·Molecular Endocrinology·Anne V McNamaraMichael R H White
Jun 14, 2019·PLoS Computational Biology·Hiroshi MomijiDavid A Rand
Jun 29, 2012·Biomedical Optics Express·C D SaunterJ M Girkin
Jan 4, 2012·Annals of the New York Academy of Sciences·Julian R E Davis

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