Real-Time Visualization of Lysosome Destruction Using a Photosensitive Toluidine Blue Nanogel

Chemistry : a European Journal
Weiqi Zhang, Ching-Hsuan Tung

Abstract

Breaking the lysosome helps its sequestered payloads access their molecular targets in cells and thus enhances the intracellular drug delivery. Current strategies for lysosomal escape involve direct physical interactions with the lipid membrane. These interactions pose a systemic toxicity and uncontrolled membrane rupture risk. Here, we report a light-detonated lysosome disruption using a hyaluronan (HA) nanogel packed with toludine blue (TB). The HA/TB nanogel is concentrated within the lysosomes. The applied light assists TB in generating reactive oxygen species and destroying the lysosome in situ, both in cells and isolated lysosomes. Real time fluorescent tracking reveals that quenched TB fluorescence recovers along with lysosome explosion, relocates to the nucleus, and is presented as a fluorescent sparkling in cells. This HA/TB, composed of all clinically approved materials, represents a biocompatible and facile strategy to "bomb" lysosomes in a spatiotemporally controlled fashion.

References

Jun 16, 1992·Mutation Research·A J DunipaceG Stookey
Mar 13, 1992·Journal of Photochemistry and Photobiology. B, Biology·A RückH Schneckenburger
Sep 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·M GèzeR Santus
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jean-François TremblayRobert Bissonnette
Jul 20, 2006·Cancer Research·Yongdoo ChoiChing-Hsuan Tung
Oct 29, 2008·Oncogene·P Boya, G Kroemer
Mar 4, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alfonso Blázquez-CastroAngeles Juarranz
Jul 6, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pål Kristian SelboKristian Berg
Nov 17, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amir K VarkouhiHidde J Haisma
Dec 28, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sashi NadanacivaYvonne Will
Jan 25, 2011·Nature Nanotechnology·Huw D SummersRachel J Errington
Aug 28, 2012·Journal of Oral and Maxillofacial Pathology : JOMFP·Gokul Sridharan, Akhil A Shankar
Oct 3, 2012·Advanced Drug Delivery Reviews·Shi XuSarah F Hamm-Alvarez
Feb 26, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Puja Paul, Gopinatha Suresh Kumar
Aug 7, 2013·Journal of Molecular Cell Biology·Hanna AppelqvistKarin Öllinger
Jan 1, 2012·Pharmaceuticals·Alfredo Erazo-OliverasJean-Philippe Pellois
Feb 3, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Benny Zhitomirsky, Yehuda G Assaraf
Dec 22, 2016·Chemical Communications : Chem Comm·Weiqi ZhangChing-Hsuan Tung
Jan 19, 2017·Photodiagnosis and Photodynamic Therapy·Fabian CieplikLamprini Karygianni
Feb 9, 2017·Biomaterials·Hyemin KimSei Kwang Hahn
Feb 23, 2017·ACS Applied Materials & Interfaces·Weiqi Zhang, Ching-Hsuan Tung
Mar 1, 2017·Nature Biotechnology·Steven F Dowdy
Mar 30, 2017·ACS Chemical Biology·Ting-Yi WangJean-Philippe Pellois

❮ Previous
Next ❯

Citations

Aug 6, 2019·Chemistry : a European Journal·Baoji DuChing-Hsuan Tung
Oct 23, 2020·Chemical Communications : Chem Comm·Zhenhua Shen, Ching-Hsuan Tung
Aug 3, 2021·Frontiers in Bioengineering and Biotechnology·Huimin WangWeiqi Zhang
Sep 24, 2020·Current Topics in Medicinal Chemistry·Menghan GaoWeiqi Zhang
Dec 29, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Changchun WenHong Liang
Jan 20, 2021·ACS Biomaterials Science & Engineering·Baoji Du, Ching-Hsuan Tung
Oct 7, 2021·ACS Nano·Weiqi ZhangChing-Hsuan Tung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal - Canadian Dental Association = Journal De L'Association Dentaire Canadienne
R A Mathews
Journal - Canadian Dental Association = Journal De L'Association Dentaire Canadienne
A Mashberg
Nihon Geka Gakkai zasshi
T Teramatsu
© 2022 Meta ULC. All rights reserved