Real-Time Visualization of Tissue Surface Biochemical Features Derived From Fluorescence Lifetime Measurements

IEEE Transactions on Medical Imaging
Dimitris GorpasLaura Marcu

Abstract

Fiber based fluorescence lifetime imaging has shown great potential for intraoperative diagnosis and guidance of surgical procedures. Here we describe a novel method addressing a significant challenge for the practical implementation of this technique, i.e., the real-time display of the quantified biochemical or functional tissue properties superimposed on the interrogated area. Specifically, an aiming beam (450 nm) generated by a continuous-wave laser beam was merged with the pulsed fluorescence excitation light in a single delivery/collection fiber and then imaged and segmented using a color-based algorithm. We demonstrate that this approach enables continuous delineation of the interrogated location and dynamic augmentation of the acquired frames with the corresponding fluorescence decay parameters. The method was evaluated on a fluorescence phantom and fresh tissue samples. Current results demonstrate that 34 frames per second can be achieved for augmenting videos of 640 × 512 pixels resolution. Also we show that the spatial resolution of the fluorescence lifetime map depends on the tissue optical properties, the scanning speed, and the frame rate. The dice similarity coefficient between the fluorescence phantom and the rec...Continue Reading

References

Aug 3, 2011·Journal of Biomedical Optics·Matthew D KellerAnita Mahadevan-Jansen
Jan 26, 2012·Annals of Biomedical Engineering·Laura Marcu
Mar 15, 2013·JSLS : Journal of the Society of Laparoendoscopic Surgeons·Ofer N GofritYoav Mintz
Mar 13, 2014·Surgical Endoscopy·Patrick PessauxJacques Marescaux
Apr 3, 2014·The Review of Scientific Instruments·Diego R YankelevichLaura Marcu
May 16, 2014·Tissue Engineering. Part C, Methods·Alexander KhmaladzeMichael D Morris
Sep 30, 2014·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·L YangE Kobayashi
Jan 30, 2015·The British Journal of Surgery·E de BoerG M van Dam
Feb 5, 2015·Journal of Biomedical Optics·Huan HuangJian Liu

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Citations

Jun 24, 2017·Methods and Applications in Fluorescence·Farzad FereidouniLaura Marcu
Nov 4, 2017·Physics in Medicine and Biology·Jennifer E PhippsLaura Marcu
Dec 31, 2017·Journal of Biomedical Optics·Stephen A BoppartHenricus J C M Sterenborg
Aug 8, 2018·Nature Nanotechnology·Yong FanFan Zhang
Dec 24, 2018·Journal of Biomedical Optics·Xiangnan ZhouLaura Marcu
Feb 17, 2019·Journal of Tissue Engineering and Regenerative Medicine·Anne K HaudenschildKyriacos A Athanasiou
Jul 14, 2020·Translational Biophotonics·Brent W WeyersLaura Marcu
Dec 4, 2019·Scientific Reports·Maria AnastasopoulouVasilis Ntziachristos
Feb 23, 2021·Critical Reviews in Analytical Chemistry·Xue-Xia JiaZhen-Chuan Fan
Apr 21, 2021·The Journal of Surgical Research·Mark MarsdenMichael J Campbell

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