Real-time whole-body imaging of an orthotopic metastatic prostate cancer model expressing red fluorescent protein

The Prostate
Meng YangRobert M Hoffman

Abstract

We describe here, a whole-body imageable spontaneous metastatic model of human prostate cancer developed by surgical orthotopic implantation (SOI) and visualized by red fluorescent protein (RFP) expression. Human prostate cancer PC-3 cells were transduced with the pLNCX2-DsRed-2-RFP retroviral vector containing the RFP and neomycin-resistance genes. A stable RFP-expressing PC-3 clone was selected in 800 microg/ml G418 in vitro and injected subcutaneously in nude mice. Stable high-level expression of RFP was maintained in the subcutaneously-growing tumors. To utilize RFP expression for metastasis studies, fragments of the subcutaneously-growing tumor, which were comprised of RFP-expressing cells, were implanted by SOI in the prostate of nude mice. Primary tumor growth, progression, and subsequent lymphatic metastases were visualized in live, intact animals in real time by whole-body RFP fluorescence imaging. In total, 100% of the experimental animals developed lymphatic metastasis, the growth of which was monitored in real time by whole-body imaging. The aggressive lymphatic metastasis in this model reflects one of the major metastatic routes of prostate cancer in human patients. Intravital RFP imaging visualized single cancer c...Continue Reading

References

Jun 17, 1992·Journal of the National Cancer Institute·R A StephensonI J Fidler
Dec 2, 1992·International Journal of Cancer. Journal International Du Cancer·X FuR M Hoffman
Sep 1, 1988·Clinical & Experimental Metastasis·D H ShevrinT E Lad
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Meng YangRobert M Hoffman

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Citations

Dec 17, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Vincent KooKate Williamson
Apr 5, 2007·Nature Protocols·Robert M Hoffman, Meng Yang
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ming ZhaoRobert M Hoffman
Apr 17, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Sylvia E Le DévédecBob van de Water
Jun 2, 2015·Expert Opinion on Drug Discovery·Kenneth C Valkenburg, Kenneth J Pienta
Dec 1, 2009·Expert Opinion on Drug Discovery·Beverly A Teicher
Sep 1, 2010·Expert Opinion on Drug Discovery·Robert M Hoffman
Oct 31, 2015·Journal of Cellular Biochemistry·Yong ZhangNaohiko Seki
Feb 28, 2007·Neoplasia : an International Journal for Oncology Research·Christian M ZechmannFabian Kiessling
Dec 24, 2015·Neoplasia : an International Journal for Oncology Research·Pierre DanhierZaver M Bhujwalla
Jun 6, 2014·ACS Medicinal Chemistry Letters·Rira WatanabeHisataka Kobayashi
Dec 7, 2006·Neuroimaging Clinics of North America·Dawid Schellingerhout
Oct 28, 2009·IUBMB Life·Jörg WiedenmannGerd Ulrich Nienhaus
Feb 21, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·G Ulrich Nienhaus, Jörg Wiedenmann
Jul 8, 2005·The Prostate·Janni MirosevichRobert J Matusik
May 4, 2011·The Prostate·Mary A HallEva M Sevick-Muraca
Aug 26, 2006·The Journal of Surgical Research·James J MezhirMitchell C Posner
Jul 11, 2020·Advanced Biosystems·Amirhossein HakamivalaLiping Tang
Mar 22, 2008·Chemistry & Biology·Simone KredelJörg Wiedenmann

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