Rearrangement of gene order in the phaCAB operon leads to effective production of ultrahigh-molecular-weight poly[(R)-3-hydroxybutyrate] in genetically engineered Escherichia coli.

Applied and Environmental Microbiology
Ayaka HiroeT Tsuge

Abstract

Ultrahigh-molecular-weight poly[(R)-3-hydroxybutyrate] [UHMW-P(3HB)] synthesized by genetically engineered Escherichia coli is an environmentally friendly bioplastic material which can be processed into strong films or fibers. An operon of three genes (organized as phaCAB) encodes the essential proteins for the production of P(3HB) in the native producer, Ralstonia eutropha. The three genes of the phaCAB operon are phaC, which encodes the polyhydroxyalkanoate (PHA) synthase, phaA, which encodes a 3-ketothiolase, and phaB, which encodes an acetoacetyl coenzyme A (acetoacetyl-CoA) reductase. In this study, the effect of gene order of the phaCAB operon (phaABC, phaACB, phaBAC, phaBCA, phaCAB, and phaCBA) on an expression plasmid in genetically engineered E. coli was examined in order to determine the best organization to produce UHMW-P(3HB). The results showed that P(3HB) molecular weights and accumulation levels were both dependent on the order of the pha genes relative to the promoter. The most balanced production result was achieved in the strain harboring the phaBCA expression plasmid. In addition, analysis of expression levels and activity for P(3HB) biosynthesis enzymes and of P(3HB) molecular weight revealed that the concen...Continue Reading

References

Feb 1, 1997·Applied Microbiology and Biotechnology·S KusakaY Doi
Jun 1, 2002·Journal of Biochemistry·Seiichi TaguchiYoshiharu Doi
Sep 5, 2003·The Biochemical Journal·Bernd H A Rehm
Jul 30, 2005·Macromolecular Bioscience·Tadahisa Iwata
Dec 30, 2006·Applied and Environmental Microbiology·Tomoko NishizakiHiroshi Yanagawa
Sep 21, 2007·Microbial Cell Factories·José Luis Báez-ViverosGuillermo Gosset
Nov 3, 2009·Metabolic Engineering·Keith E J TyoGregory Stephanopoulos
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Han N LimRazika Hussein

❮ Previous
Next ❯

Citations

May 15, 2013·Applied Biochemistry and Biotechnology·Johanna Katherine BocanegraJose Gregório Cabrera Gomez
Apr 2, 2013·Applied and Environmental Microbiology·Daniel HeinrichAlexander Steinbüchel
May 27, 2014·Applied Microbiology and Biotechnology·Björn AndreeßenAlexander Steinbüchel
Jan 8, 2013·Journal of Bioscience and Bioengineering·Naoya KataokaJunichi Kato
Aug 21, 2013·Biotechnology Advances·Prasun KumarVipin C Kalia
May 20, 2015·Biochimica Et Biophysica Acta·Razika HusseinHan N Lim
Jun 13, 2015·Indian Journal of Microbiology·Mamtesh SinghVipin C Kalia
Mar 19, 2013·Current Opinion in Biotechnology·Namita BhanMattheos A G Koffas
Dec 17, 2014·Frontiers in Bioengineering and Biotechnology·Richard KelwickPaul Freemont
Dec 21, 2014·Applied Microbiology and Biotechnology·Tomoko YoshinoTsuyoshi Tanaka
Jun 18, 2017·Applied Microbiology and Biotechnology·Dan LiGuo-Qiang Chen
Apr 7, 2018·Applied Microbiology and Biotechnology·Yilin RenGuo-Qiang Chen
Sep 1, 2018·FEMS Microbiology Letters·Kenji OkanoAkihiko Kondo
Dec 18, 2013·Applied and Environmental Microbiology·Manami HyakutakeTakeharu Tsuge
Mar 4, 2014·Applied and Environmental Microbiology·Kazunori UshimaruTakeharu Tsuge
Jan 29, 2019·Bioinformatics·Huy NguyenIddo Friedberg
Mar 11, 2019·World Journal of Microbiology & Biotechnology·Tatiana ThomasStéphane Bruzaud
Aug 1, 2018·Journal of Molecular Evolution·Gurusamy Kutralam-MuniasamyFermín Pérez-Guevara
Apr 23, 2020·Frontiers in Bioengineering and Biotechnology·Camila UtsunomiaManfred Zinn
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Richard J R KelwickPaul S Freemont
Jul 18, 2019·International Journal of Biological Macromolecules·Ken HaradaTakeharu Tsuge
Nov 18, 2020·International Journal of Biological Macromolecules·Ayaka HiroeSeiichi Taguchi
Jan 29, 2021·Bioresource Technology·Abhishek Dutt TripathiKianoush Khosravi Darani
Mar 18, 2021·Critical Reviews in Biotechnology·Kai-Hee HuongA A Amirul
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Albert Gyapong AduheneChangli Liu
Jun 22, 2021·Frontiers in Bioengineering and Biotechnology·Thanawat BoontipSuchada Chanprateep Napathorn
Sep 18, 2013·ACS Chemical Biology·Ayaka HiroeTakeharu Tsuge

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.