Rearrangement of the 16S precursor subunits is essential for the formation of the active 20S proteasome

Biophysical Journal
Srinivas MullapudiPawel A Penczek

Abstract

Proteasome-dependent proteolysis is essential for a number of key cellular processes and requires a sophisticated biogenesis pathway to function. Here, we have arrested the assembly process in its dynamic progression at the short-lived 16S state. Structural analysis of the 16S proteasome precursor intermediates by electron microscopy, and single particle analysis reveals major conformational changes in the structure of the beta-ring in comparison with one-half of the 20S proteasome. The individual beta-subunits in the 16S precursor complex rotate with respect to their positions in the x-ray crystallographic structure of the fully assembled 20S. This rearrangement results in a movement of the catalytic residue threonine-1 from the protected location in 16S precursor complexes to a more exposed position in the 20S structure. Thereby, our findings provide a molecular explanation for the structural rearrangements necessary for the dimerization of two 16S precursor complexes and the subsequent final maturation to active 20S proteasomes.

References

Jan 1, 1992·Ultramicroscopy·P PenczekJ Frank
Nov 1, 1994·Nature Structural Biology·P ZwicklW Baumeister
Mar 1, 1995·Nature Structural Biology·T Wenzel, W Baumeister
Aug 1, 1996·Nature·E SeemullerW Baumeister
Oct 6, 1997·The Journal of Biological Chemistry·W HeinemeyerD H Wolf
Dec 31, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Schmidt, P M Kloetzel
Dec 31, 1997·FEBS Letters·F ZühlW Baumeister
Jan 27, 1998·Journal of Structural Biology·A J KosterW Baumeister
Jun 27, 1998·Journal of Molecular Biology·L DitzelM Groll
Mar 2, 1999·Journal of Structural Biology·J MayrW Baumeister
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M GrollR Huber
May 19, 2000·The Journal of Biological Chemistry·D J KingsburyR A Colbert
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
Mar 5, 2003·Journal of Molecular Biology·Michael GrollRobert Huber
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Michael Groll, Robert Huber
Apr 11, 2003·Reviews of Physiology, Biochemistry and Pharmacology·E KrügerP-M Kloetzel

❮ Previous
Next ❯

Citations

Jul 25, 2006·Journal of Structural Biology·Chao YangJoachim Frank
Feb 28, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Saul R Powell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.