Reassembly of functional nucleoli following in situ unraveling by low-ionic-strength treatment of cultured mammalian cells

Experimental Cell Research
O V ZatsepinaM F Trendelenburg

Abstract

In order to determine the most persistent components of the nucleolus that might serve as "core" nucleolar elements, we studied the reactivity of nucleoli in living mammalian cells subjected to hypotonic buffer saline followed by the incubation of the cells in an isotonic medium. To document as precisely as possible the fine structural changes which occurred, the cells were examined by video-enhanced optical microscopy, fluorescence confocal laser scanning microscopy, and electron microscopy combined with cytochemistry. Light microscopic autoradiography was used to demonstrate the transcriptional characteristics of the reassembled nucleoli. It was shown that all the major compartments of the intact nucleolus could be substantially affected by reduction of the osmolarity of the environmental media. The dynamic events of the nucleolar unraveling in low-salt buffers occurred in the following order: dispersion of the nucleolar pars granulosa, disassociation of the fibrillar complexes into discrete fibrillar centers (FCs) and the dense fibrillar component (DFC), and the almost complete unraveling of the DFC and FCs. At the terminal stages of nucleolar dispersion, the nuclear interior was mainly composed of a loose filamentous meshwo...Continue Reading

References

Nov 1, 1978·Experimental Cell Research·A Lepoint, G Goessens
Nov 1, 1992·Experimental Cell Research·P HozákD Hernandez-Verdun
Sep 1, 1992·The Journal of Cell Biology·P BellU Scheer
Mar 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·A P DybanY S Chentsov
Jan 1, 1990·Biology of the Cell·P HozákZ Likovský
Dec 1, 1991·Chromosoma·D FischerU Scheer
Dec 1, 1991·Experimental Cell Research·R L Ochs, K Smetana
Jun 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J R WagleB M Sanborn
Jan 1, 1985·Biology of the Cell·R L OchsH Busch
Sep 1, 1988·Cell Biology International Reports·M F TrendelenburgH Spring
Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·R D Allen
Jan 1, 1985·Chromosoma·R L OchsH Busch
Jun 1, 1985·Molecular and Cellular Biology·R I BollaD Schlessinger
Jan 1, 1983·The Journal of Cell Biology·W C Earnshaw, U K Laemmli
Aug 1, 1993·The Journal of Cell Biology·G M Trimbur, C J Walsh
Jan 1, 1995·The Journal of Cell Biology·M A Garcia-BlancoM P Sheetz
Jun 1, 1994·Current Opinion in Cell Biology·U Scheer, D Weisenberger
May 1, 1993·The American Journal of Physiology·S Medrano, E Gruenstein
Jan 1, 1995·Annual Review of Cell and Developmental Biology·P J Shaw, E G Jordan
Aug 1, 1996·Histochemistry and Cell Biology·M F TrendelenburgH Spring

❮ Previous
Next ❯