Reassessing the organization of the UL42-UL43 region of the human cytomegalovirus strain AD169 genome

Virology
E S MocarskiJ M Brown

Abstract

A polymorphism in the UL42-UL43 region of the human cytomegalovirus genome has been characterized by nucleotide sequence analysis, revealing a 929-bp insertion following nt 54,612 relative to the published strain AD169-UK genome sequence (M.S. Chee et al., 1990, Curr. Top. Microbiol Immunol. 154, 125-170). Although AD169-UK exhibited polymorphism in this genomic region, other CMV strains (Towne, Toledo, and AD169-ATCC) carried only the newly characterized longer form. The additional sequence altered the assignment of UL42 and UL43 open reading frames. UL42 decreased in size from 157 to 125 codons, retaining 76 of the previously reported carboxyl terminal codons, and UL43 increased in size from 187 to 423 codons, retaining 185 of the previously reported amino terminal codons. This additional sequence makes UL43 a more conserved betaherpesvirus US22 family member. Only AD169-UK exhibited restriction fragment length polymorphism in this region, suggesting that a deletion occurred during the propagation of this strain in cell culture. The additional sequence should be considered a bona fide part of the cytomegalovirus genome and the AD169 genome size should be corrected to 230,283 bp.

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Citations

Mar 14, 2003·Journal of Virology·Isidore RigoutsosThomas Shenk
Aug 4, 2010·The Journal of Clinical Investigation·Richard J StantonGavin W G Wilkinson
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eain MurphyThomas E Shenk
Nov 12, 2015·The Journal of General Virology·Tetsuo KoshizukaTatsuo Suzutani
Sep 10, 1999·Reviews in Medical Virology·K Umene
Jun 26, 2009·The Journal of General Virology·Amanda J BradleyAndrew J Davison
Nov 26, 2010·Archives of Virology·R NascimentoR M E Parkhouse
Aug 15, 2003·The Journal of General Virology·Brian P McSharryGavin W G Wilkinson
Jan 21, 2003·The Journal of General Virology·Andrew J DavisonGary S Hayward
May 29, 2003·The Journal of General Virology·Giuseppe GernaM Grazia Revello
Apr 24, 2004·The Journal of General Virology·Aidan DolanAndrew J Davison

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