PMID: 1666184Sep 1, 1991Paper

Reassessment of plasma angiotensins measurement: effects of protease inhibitors and sample handling procedures

Peptides
K KoharaC M Ferrario

Abstract

Characterization of C- and N-terminal forms of angiotensin (Ang) peptides mandated assessment of methods to determine plasma levels. 125I-Ang I, 125I-Ang II, and 125I-Ang(1-7) were added to blood samples in the presence of protease inhibitors. Ethylenediaminetetraacetic acid (EDTA) inhibited the conversion of 125I-Ang I to 125I-Ang II. o-Phenanthroline and EDTA (EDTA + o-Ph) did not eliminate [des-Asp1] fragments or 125I-Ang(1-7). The combination of EDTA + o-Ph and pepstatin A or 4-(chloromercuri) benzoic acid (PCMB) significantly reduced 125I-Ang(1-7) generation. Only PCMB plus EDTA + o-Ph eliminated [des-Asp1] fragments. Authentic plasma values of Ang peptides require the correct choice of protease inhibitors.

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Citations

Jan 4, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Robert C Speth, Vardan T Karamyan
Sep 1, 1993·Peptides·K KoharaC M Ferrario
Jul 1, 1996·The International Journal of Biochemistry & Cell Biology·L C KatwaK T Weber
May 11, 1999·Regulatory Peptides·K YamadaC M Ferrario
Mar 17, 2004·Journal of Pharmaceutical and Biomedical Analysis·Hervé VollandChristophe Creminon
Mar 26, 1998·Journal of Cardiovascular Pharmacology·S N IyerC M Ferrario
Sep 11, 1998·Journal of Cardiovascular Pharmacology·N J BrownA Adam
Mar 9, 1999·Journal of Cardiovascular Pharmacology·W B StrawnC M Ferrario
Sep 18, 2002·Kidney International·Carlos M FerrarioDebra I Diz
Dec 22, 2006·The Journal of Pharmacology and Experimental Therapeutics·Jean LeFebvreNancy J Brown
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·B G Zimmerman, E W Dunham
Nov 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·W Jeffrey PettyFrank M Torti
Jan 17, 2012·Circulation. Arrhythmia and Electrophysiology·Hossam I MustafaDavid Robertson
Dec 1, 2009·Therapeutic Advances in Cardiovascular Disease·Jasmina VaragicCarlos M Ferrario
Jul 1, 1997·Journal of Hypertension·K B BrosnihanC M Ferrario
Jan 27, 2011·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Hossam I MustafaSatish R Raj
Feb 23, 2010·Regulatory Peptides·Felicia M RabeyRobert C Speth
Jun 17, 2004·British Journal of Haematology·William B StrawnCarlos M Ferrario
Feb 20, 2007·Brain Research·Vardan T Karamyan, Robert C Speth
Apr 1, 1997·American Journal of Obstetrics and Gynecology·J R StanleyJ C Rose
Nov 28, 2013·Therapeutic Advances in Cardiovascular Disease·Carlos M FerrarioRonald D Smith
Sep 10, 2017·Journal of Cellular Physiology·Sarfaraz AhmadHao Wang
Mar 17, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Edilamar M OliveiraJosé E Krieger
Jun 9, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·N J BrownD E Vaughan
Feb 15, 1995·Circulation·T KitazonoD D Heistad
Feb 4, 1998·Hypertension·S K GandhiN J Brown
Sep 18, 1998·Hypertension·K YamadaC M Ferrario

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