Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis.

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Alex HorsleyD J Thornton

Abstract

There is conflicting evidence about the importance of airway mucins (MUC5AC and MUC5B) in determining physical properties of sputum in cystic fibrosis (CF). We studied the effects of endogenous degradation of mucins on CF sputum elasticity and apparent mucin concentrations. Elastic shear moduli (G') and mucin concentrations in sputum of 12 CF patients were measured before and after incubation at 37°C for 60min. G' fell from a median of 5.98 to 4.70Pa (p=0.01). There were significant falls in MUC5AC (8.2 to 5.2μg/ml, p=0.02) and MUC5B (17.3 to 12.5μg/ml, p=0.02) over the same period, and associated decrease in molecular weight and size. Sputum is not inert and degradation reduces apparent mucin concentrations and sputum elasticity. Even if care is taken to process samples rapidly, sputum may therefore differ from secretions retained in airways. Previous studies may have underestimated the role of mucins in CF sputum.

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Citations

Apr 2, 2014·The International Journal of Biochemistry & Cell Biology·Camille EhreDavid J Thornton
Nov 2, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gregg A DuncanJung Soo Suk
Nov 16, 2018·Annals of the American Thoracic Society·David J ThorntonCaroline Ridley
Sep 14, 2018·American Journal of Respiratory and Critical Care Medicine·Camille EhreRichard C Boucher
Nov 26, 2019·American Journal of Respiratory and Critical Care Medicine·Kathryn A RamseyMichael A McGuckin
Mar 20, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Caroline RidleyDavid J Thornton
Aug 15, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Daniela Peneda PachecoPaola Petrini
May 24, 2019·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Vera BrinksBianca Matthee

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Methods Mentioned

BETA
electrophoresis
density gradient centrifugation
size-exclusion chromatography
rheology

Software Mentioned

Prism
GraphPad

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