Reassessment of the Role of Rapid Antigen Detection Tests in Diagnosis of Invasive Group A Streptococcal Infections

Journal of Clinical Microbiology
Vincent GazzanoGhislaine Descours

Abstract

Rapid antigen detection tests (RADTs) for group A streptococci (GAS) are widely used for diagnosing acute pharyngitis, which has led to a considerable reduction in antibiotic prescriptions over the past decade. Beyond this intended use, their reassessment on invasive samples may be relevant in the management of life-threatening GAS infections. To this end, we evaluated the performances of three RADTs, culture, GAS PCR, and 16S rRNA gene PCR assays, and compared them with a composite gold standard (GAS-PCR assay and/or culture) for the diagnosis of severe GAS infection. A total of 192 specimens from deep-tissue (mostly normally sterile) sites enriched for 75 GAS-positive samples were enrolled in the study. The three evaluated RADTs showed sensitivities ranging from 88.0% to 94.7% versus 98.7% for GAS PCR, 84% for 16S rRNA gene PCR, and 77.3% for culture. The sensitivities of the ImmunoCardSTAT! Strep A test (Meridian Bioscience) and the NADAL Strep A strip (Nal Von Minden) were similar to that of GAS PCR (P= 0.25 and 0.03, respectively) and higher than that of culture (P= 0.001 and 0.006, respectively), whereas the SD Bioline Strep A test strip (Standard Diagnostics) showed a performance similar to that of culture (P= 0.02). The...Continue Reading

References

Oct 1, 1987·Burns, Including Thermal Injury·A A AzmyL Robertson
Feb 8, 2003·Journal of Clinical Microbiology·Valérie GauduchonFrançois Vandenesch
Aug 13, 2003·The Pediatric Infectious Disease Journal·Herbert W CleggUNKNOWN Presbyterian Pediatric Research Group
Apr 8, 2004·JAMA : the Journal of the American Medical Association·Warren J McIsaacDonald E Low
Jun 6, 2007·The Medical Journal of Australia·Kerry-Ann F O'GradyJonathan R Carapetis
Sep 7, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rosalyn E O'LoughlinUNKNOWN Active Bacterial Core Surveillance Team
May 9, 2008·Journal of Clinical Microbiology·Theresa L LamagniAftab Jasir
Oct 30, 2009·Pediatric Emergency Care·Steve AyanruohToussaint Reynolds
May 31, 2011·The British Journal of General Practice : the Journal of the Royal College of General Practitioners·Carl LlorJosep Maria Cots
Sep 3, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C PlainvertUNKNOWN CNR-Strep network
May 23, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P BidetE Bingen
May 25, 2012·Journal of Clinical Microbiology·Xiaotian ZhengStanford T Shulman
Dec 5, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N ToepfnerM Hufnagel
Jan 24, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J F CohenR Cohen
Mar 19, 2013·Journal of Microbiological Methods·Juan Jose PicazoUNKNOWN Heracles Study Group
Aug 13, 2013·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·M ParkA Martinot
May 3, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jonathan R CarapetisRoss Andrews
Jun 15, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Anna LinnérAnna Norrby-Teglund
Oct 15, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert CohenPhilippe Bidet
Dec 3, 2014·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·C PlainvertC Poyart
Dec 3, 2014·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·A WollnerR Cohen
Sep 1, 2005·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·T L Lamagni Strep-Euro

❮ Previous
Next ❯

Citations

Feb 16, 2017·Annals of Thoracic Medicine·Jaffar A Al-Tawfiq, Amel H Alawami
Jan 8, 2017·Enfermedades infecciosas y microbiología clínica·José María Marimón, José María Navarro-Marí
Apr 15, 2018·Scientific Reports·Robert J EdwardsShiranee Sriskandan
Feb 2, 2021·Frontiers in Pediatrics·Ana Barrés-FernándezCecilia Martínez-Costa
Jan 20, 2021·ACS Biomaterials Science & Engineering·Tobias MieslerLorenz Meinel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.