Rebamipide attenuates 5-Fluorouracil-induced small intestinal mucositis in a mouse model

Biological & Pharmaceutical Bulletin
Hyun Jin KimJong Hun Lee

Abstract

5-Fluorouracil (5-FU)-induced intestinal mucositis is one of the most common morbidities in chemotherapy and involves the reactive oxygen species (ROS) system, apoptosis, and inflammatory cytokines. Rebamipide exerts a mucosal-protective effect, mediated through several mechanisms. The aim of this study was to evaluate the effects of rebamipide in 5-FU-induced mouse small-intestinal mucositis. BALB/c mice were assigned randomly to four groups; (1) control group (n=10; receiving saline orally for 6 d), (2) rebamipide group (n=10; 150 mg/kg rebamipide for 6 d orally), (3) 5-FU group (n=10; 30 mg/kg 5-FU for 5 d, intraperitoneally (i.p.)), and (4) rebamipide +5-FU group (n=10; 150 mg/kg rebamipide for 6 d orally and 30 mg/kg 5-FU for 5 d, i.p.). Body weights and diarrhea scales were assessed. At day 5, the mice were sacrificed. Small intestinal tissue was used for: (1) hematoxylin and eosin (HE) staining for determination of small intestinal villi height, (2) terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay, (3) immunohistochemistry for inducible nitric oxide synthase (iNOS), F4/80, and transforming growth factor (TGF)-β1, (4) measurement of serum and tissue GSH levels, and (...Continue Reading

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Citations

Apr 17, 2019·Pharmaceuticals·João Antônio Leal de MirandaGilberto Santos Cerqueira
Mar 30, 2019·Current Opinion in Supportive and Palliative Care·Hannah R WardillAndrea M Stringer
Jun 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lázaro de Sousa FidelesGilberto Santos Cerqueira
Jan 16, 2020·Pharmaceuticals·João Antônio Leal de MirandaGilberto Santos Cerqueira
Oct 13, 2020·Frontiers in Microbiology·Viviane Lima BatistaMariana Martins Drumond
Jan 10, 2021·Chinese Journal of Integrative Medicine·Meng-Xuan GuiJiu-Mao Lin
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Si ZhangChengliang Zhang

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