RecA-like domain 2 of DNA-dependent ATPase A domain, a SWI2/SNF2 protein, mediates conformational integrity and ATP hydrolysis

Bioscience Reports
Ritu BansalRohini Muthuswami

Abstract

ATP-dependent chromatin remodeling proteins use the energy released from ATP hydrolysis to reposition nucleosomes in DNA-dependent processes. These proteins are classified as SF2 helicases. SMARCAL1, a member of this protein family, is known to modulate both DNA repair and transcription by specifically recognizing DNA molecules possessing double-strand to single-strand transition regions. Mutations in this gene cause a rare autosomal recessive disorder known as Schimke Immuno-Osseous Dysplasia (SIOD).Structural studies have shown that the ATP-dependent chromatin remodeling proteins possess two RecA-like domains termed as RecA-like domain 1 and RecA-like domain 2. Using Active DNA-dependent ATPase A domain (ADAAD), the bovine homolog of SMARCAL1, as a model system we had previously shown that the RecA-like domain 1 containing helicase motifs Q, I, Ia, II, and III are sufficient for ligand binding; however, the Rec A-like domain 2 containing motifs IV, V, and VI are needed for ATP hydrolysis. In the present study, we have focused on the motifs present in the RecA-like domain 2. Our studies demonstrate that the presence of an aromatic residue in motif IV is needed for interaction with DNA in the presence of ATP. We also show that ...Continue Reading

References

Oct 1, 1996·Nucleic Acids Research·E Richmond, C L Peterson
Nov 28, 1996·Nature·H S SubramanyaD B Wigley
Jun 1, 1997·Nature Structural Biology·N YaoP C Weber
Sep 29, 1999·Genes & Development·R E Kingston, G J Narlikar
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·J M CaruthersD B McKay
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M StoryJ N Abelson
Aug 3, 2001·Journal of Virology·C L TaiD S Chen
Jan 19, 2002·Nature Genetics·Cornelius F BoerkoelDavid W Stockton
Feb 13, 2002·Current Opinion in Structural Biology·Jonathan M Caruthers, David B McKay
Jul 24, 2004·EMBO Reports·Efrosyni PapanikouAnastassios Economou
Apr 5, 2005·Nature Structural & Molecular Biology·Nicolas H ThomäNikola P Pavletich
Jun 2, 2006·Nucleic Acids Research·Andrew FlausTom Owen-Hughes
Apr 15, 2008·Nature Structural & Molecular Biology·Jack A VincentToshio Tsukiyama
Oct 2, 2009·Genes & Development·Timur YusufzaiJames T Kadonaga
Oct 21, 2009·The Journal of Biological Chemistry·Lisa PostowHironori Funabiki
Jan 30, 2010·Nature·Lena Ho, Gerald R Crabtree
May 12, 2010·Current Opinion in Structural Biology·Margaret E Fairman-WilliamsEckhard Jankowsky
Mar 2, 2011·Cell Research·Diana C Hargreaves, Gerald R Crabtree
Aug 4, 2011·The FEBS Journal·Andrew Flaus, Tom Owen-Hughes
Apr 19, 2012·Biochemistry·Macmillan NongkhlawRohini Muthuswami
Jul 22, 2015·The FEBS Journal·Meghna GuptaRohini Muthuswami

❮ Previous
Next ❯

Citations

Jan 23, 2019·Journal of Cellular Physiology·Rohini MuthuswamiJoel W Hockensmith
Aug 6, 2020·IUBMB Life·Ritu BansalRohini Muthuswami
Feb 23, 2020·Genes·Sonia Vidushi Gupta, Kristina Hildegard Schmidt

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
fluorescence studies
fluorescence spectroscopy
fluorescence

Software Mentioned

Clustal
ADAAD
Clustal W

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.