RECA plays a dual role in the maintenance of chloroplast genome stability in Physcomitrella patens

The Plant Journal : for Cell and Molecular Biology
Masaki OdaharaYasuhiko Sekine

Abstract

Chloroplast DNA (cpDNA) encodes essential genes for chloroplast functions, including photosynthesis. Homologous recombination occurs frequently in cpDNA; however, its significance and underlying mechanism remain poorly understood. In this study, we analyzed the role of a nuclear-encoded chloroplast-localized homolog of RecA recombinase, which is a key factor in homologous recombination in bacteria, in the moss Physcomitrella patens. Complete knockout (KO) of the P. patens chloroplast RecA homolog RECA2 caused a modest growth defect and conferred sensitivity to methyl methanesulfonate and UV. The KO mutant exhibited low recovery of cpDNA from methyl methanesulfonate damage, suggesting that RECA2 knockout impairs repair of damaged cpDNA. The RECA2 KO mutant also exhibited reduced cpDNA copy number and an elevated level of cpDNA molecule resulting from aberrant recombination between short dispersed repeats (13-63 bp), indicating that the RECA2 KO chloroplast genome was destabilized. Taken together, these data suggest a dual role for RECA2 in the maintenance of chloroplast genome stability: RECA2 suppresses aberrant recombination between short dispersed repeats and promotes repair of damaged DNA.

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H CeruttiA T Jagendorf
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Aug 11, 1998·Trends in Biochemical Sciences·P Baumann, S C West
Oct 3, 1999·Progress in Nucleic Acid Research and Molecular Biology·M M Cox
Mar 16, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T NishiyamaM Hasebe
Nov 20, 2001·Nucleic Acids Research·S KurtzR Giegerich
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Charles L LimoliJames E Cleaver
Jun 5, 2002·Annual Review of Biochemistry·Shelley L Lusetti, Michael M Cox
Nov 18, 2003·Annual Review of Genetics·Justin Courcelle, Philip C Hanawalt
Jan 22, 2004·Nature Reviews. Genetics·Jeremy N TimmisWilliam Martin
Feb 28, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ralph A Bungard
Apr 7, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Geoffrey S BriggsRobert G Lloyd
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R Kolodner, K K Tewari
Jun 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhenguo LinHong Ma
Feb 13, 2007·Current Opinion in Plant Biology·Ralph S QuatranoDavid J Cove
May 1, 2007·The Plant Cell·Vikas ShedgeSally A Mackenzie
Nov 27, 2007·The Plant Journal : for Cell and Molecular Biology·Obed W OdomDavid L Herrin
Dec 7, 2007·Nucleic Acids Research·William C Burhans, Martin Weinberger
May 8, 2008·Bioscience, Biotechnology, and Biochemistry·Takayuki InouyeYasuhiko Sekine
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexandre MaréchalNormand Brisson
Feb 4, 2010·Methods : a Companion to Methods in Enzymology·Senyene E HunterJoel N Meyer
Apr 22, 2010·Journal of Experimental Botany·Beth A RowanArnold J Bendich
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Taegun KwonDavid L Herrin
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Beverley R Green
Mar 15, 2012·Plant Physiology·Marie Miller-MessmerJosé M Gualberto

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Citations

Jan 18, 2020·International Journal of Molecular Sciences·Nicolas ChevignyJosé Manuel Gualberto
Apr 14, 2017·The Plant Journal : for Cell and Molecular Biology·Masaki OdaharaYasuhiko Sekine
Oct 4, 2019·PeerJ·Dyfed Lloyd EvansDiego M Riaño Pachón

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