PMID: 8610174Apr 2, 1996Paper

RecA protein stimulates homologous recombination in plants

Proceedings of the National Academy of Sciences of the United States of America
B ReissJ Schell

Abstract

A number of RecA-like proteins have been found in eukaryotic organisms. We demonstrate that the prokaryotic recombination protein RecA itself is capable of interacting with genomic homologous DNA in somatic plant cells. Resistance to the DNA crosslinking agent mitomycin C requires homologous recombination as well as excision repair activity. Tobacco protoplasts expressing a nucleus-targeted RecA protein were at least three times as efficient as wild-type cells in repairing mitomycin C-induced damage. Moreover, homologous recombination at a defined locus carrying an endogenous nuclear marker gene was stimulated at least 10-fold in transgenic plant cells expressing nucleus-targeted RecA. The increase in resistance to mitomycin C and the stimulation of intrachromosomal recombination demonstrate that Escherichia coli RecA protein is functional in genomic homologous recombination in plants, especially when targeted to the plant nucleus.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A Sancar, W D Rupp
Feb 8, 1991·Cell·P A Silver
Jul 1, 1990·Plant Molecular Biology·F GuerineauP Mullineaux
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·A I Roca, M M Cox
Jan 1, 1986·Annual Review of Cell Biology·C Dingwall, R A Laskey
Jan 1, 1994·Annual Review of Biochemistry·S C Kowalczykowski, A K Eggleston
Apr 1, 1994·Current Opinion in Genetics & Development·H J Dunderdale, S C West
Aug 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·V N IYER, W SZYBALSKI

❮ Previous
Next ❯

Citations

May 18, 2001·Mutation Research·R PaulP Lestienne
Apr 5, 2001·Trends in Plant Science·S Kumar, M Fladung
Feb 25, 2003·Trends in Plant Science·Anne B Britt, Gregory D May
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·M HaninJ Paszkowski
Jan 27, 2011·Nucleic Acids Research·Hsin-Kai Liao, Jeffrey J Essner
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·G ShalevA A Levy
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·B Hohn, H Puchta
May 5, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A KönigJ M Wal
Aug 28, 2003·Journal of Plant Physiology·Holger Puchta
Oct 21, 1999·Molecular Genetics and Metabolism·V A Lanzov
Mar 18, 2005·Genetika·R A KomakhinA A Zhuchenko
Sep 11, 2004·FEBS Letters·Hong-Qing LiShigeru Iida
Jan 21, 2017·Frontiers in Plant Science·Mihaly HorvathBernd Reiss
Jul 18, 2020·BMC Plant Biology·Abdellah BarakateClaire Halpin
Oct 8, 2020·Frontiers in Genetics·Prabha VermaVineet Gaur
Feb 9, 2006·Chemical Reviews·Seisuke Kimura, Kengo Sakaguchi
Feb 7, 2002·Journal of Molecular Biology·Silvia AyoraJuan C Alonso

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved