Recellularization of rat liver scaffolds by human liver stem cells

Tissue Engineering. Part a
Victor Navarro-TablerosGiovanni Camussi

Abstract

In the present study, rat liver acellular scaffolds were used as biological support to guide the differentiation of human liver stem-like cells (HLSC) to hepatocytes. Once recellularized, the scaffolds were maintained for 21 days in different culture conditions to evaluate hepatocyte differentiation. HLSC lost the embryonic markers (alpha-fetoprotein, nestin, nanog, sox2, Musashi1, Oct 3/4, and pax2), increased the expression of albumin, and acquired the expression of lactate dehydrogenase and three subtypes of cytochrome P450. The presence of urea nitrogen in the culture medium confirmed their metabolic activity. In addition, cells attached to tubular remnant matrix structures expressed cytokeratin 19, CD31, and vimentin. The rat extracellular matrix (ECM) provides not only a favorable environment for differentiation of HLSC in functional hepatocytes (hepatocyte like) but also promoted the generation of some epithelial-like and endothelial-like cells. When fibroblast growth factor-epidermal growth factor or HLSC-derived conditioned medium was added to the perfusate, an improvement of survival rate was observed. The conditioned medium from HLSC potentiated also the metabolic activity of hepatocyte-like cells repopulating the ac...Continue Reading

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Citations

Aug 10, 2016·Stem Cells and Development·Sharmila FagooneeFiorella Altruda
Apr 11, 2017·Liver International : Official Journal of the International Association for the Study of the Liver·Fanwei MengDieter Broering
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May 14, 2021·Frontiers in Cell and Developmental Biology·Stefania BrunoGiovanni Camussi
Aug 28, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Nicola De StefanoRenato Romagnoli

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Methods Mentioned

BETA
confocal microscopy
biopsies

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