Recent advances in discovery, heterologous expression, and molecular engineering of cyclodextrin glycosyltransferase for versatile applications

Biotechnology Advances
Ruizhi HanJian Chen

Abstract

Cyclodextrin glycosyltransferase (CGTase) is an important enzyme with multiple functions, in particular the production of cyclodextrins. It is also widely applied in baking and carbohydrate glycosylation because it participates in various types of catalytic reactions. New applications are being found with novel CGTases being isolated from various organisms. Heterologous expression is performed for the overproduction of CGTases to meet the requirements of these applications. In addition, various directed evolution techniques have been applied to modify the molecular structure of CGTase for improved performance in industrial applications. In recent years, substantial progress has been made in the heterologous expression and molecular engineering of CGTases. In this review, we systematically summarize the heterologous expression strategies used for enhancing the production of CGTases. We also outline and discuss the molecular engineering approaches used to improve the production, secretion, and properties (e.g., product and substrate specificity, catalytic efficiency, and thermal stability) of CGTase.

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Citations

Feb 18, 2015·Archaea : an International Microbiological Journal·Ying SunJinliang Liu
Apr 21, 2017·Chembiochem : a European Journal of Chemical Biology·Rama Krishna Gudiminchi, Bernd Nidetzky
Jun 14, 2018·Anais Da Academia Brasileira De Ciências·Andrea C S M GomesAndre C Amaral
Jun 2, 2017·Bioprocess and Biosystems Engineering·Keren Hapuque PinheiroFlavio Faria de Moraes
Jul 1, 2017·Journal of Industrial Microbiology & Biotechnology·Ruizhi HanYe Ni
Jul 23, 2018·Applied Microbiology and Biotechnology·Xingtong ZhuWanmeng Mu
Dec 4, 2020·Glycobiology·Kazi Zubaida Gulshan AraEva Nordberg Karlsson
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Ming MiaoJames N BeMiller
May 15, 2021·Critical Reviews in Biotechnology·Peng ZhangJin-Song Shi

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