Recent advances in optical microscopic methods for single-particle tracking in biological samples

Analytical and Bioanalytical Chemistry
Yuanyuan MaLehui Xiao

Abstract

With the rapid development of optical microscopic techniques, explorations on the chemical and biological properties of target objects in biological samples at single-molecule/particle level have received great attention recently. In the past decades, various powerful techniques have been developed for single-particle tracking (SPT) in biological samples. In this review, we summarize the commonly used optical microscopic methods for SPT, such as total internal reflection fluorescence microscopy (TIRFM), super-resolution fluorescence microscopy (SRM), dark-field optical microscopy (DFM), total internal reflection scattering microscopy (TIRSM), and differential interference contrast microscopy (DICM). We then discuss the image processing and data analysis methods, including particle localization, trajectory reconstruction, and diffusion behavior analysis. The application of SPT on the cell membrane, within the cell, and the cellular invading process of viruses are introduced. Finally, the challenges and prospects of optical microscopic technologies for SPT are delineated.

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Citations

Oct 1, 2020·Computational and Structural Biotechnology Journal·Erik Meijering
Feb 12, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Boris I GodoySean B Andersson
Mar 10, 2021·Plant Methods·Saoirse AmarteifioGiovanni Sena
Nov 22, 2019·Analytical Chemistry·Zhongju YeLehui Xiao
Jan 18, 2020·Chemical Reviews·Shu-Lin LiuDai-Wen Pang

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