Recent advances in the analysis of macromolecular interactions using the matrix-free method of sedimentation in the analytical ultracentrifuge

Biology
Stephen E HardingGary G Adams

Abstract

Sedimentation in the analytical ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or "hetero"-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10-12 M to 10-1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions.

References

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Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ivano BertiniPaola Turano
Jul 26, 2011·Angewandte Chemie·Thomas HeinzeStephen E Harding
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Mar 31, 2015·Carbohydrate Polymers·Fahad M AlmutairiStephen E Harding

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Citations

Feb 2, 2020·European Biophysics Journal : EBJ·Maximilian J UttingerWolfgang Peukert
Aug 7, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Maximilian J UttingerWolfgang Peukert

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Methods Mentioned

BETA
light scattering
dynamic light scattering
x-ray scattering
acetylation
NMR

Software Mentioned

SEDFIT
ELLIPS1
MULTISIG
MULTIG
MSTAR

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