Recent advances in the engineering and application of streptavidin-like molecules

Applied Microbiology and Biotechnology
Quan LeSheldon Park

Abstract

Streptavidin (SA), and other related proteins, has been isolated from a wide range of organisms, including bacteria, fungi, frogs, fish, and birds. Although their original function is not well understood, they have found an important place in biotechnology based on their unique ability to bind biotin molecules with high affinity and specificity. The SA-biotin interaction is robust and easy to incorporate into different designs, and as such, it is used when reliable molecule interaction is needed under poorly controlled experimental conditions. There are continued efforts to engineer these proteins to modulate their size, valency, and affinity, since the optimum molecular properties vary depending on individual applications. This review will describe recent developments in streptavidin engineering to meet these requirements, including those that form novel oligomeric states, e.g., a monomer, have fewer functional biotin-binding sites, or bind biotin with reduced affinity. We also examine various reported applications of both natural or engineered SA constructs in cell biology, biochemistry, genetics, synthetic chemistry, cancer therapy, drug delivery, and nanotechnology to illustrate the breadth of modern science that is advance...Continue Reading

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Citations

May 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nicolò SantiLouis Y P Luk
May 8, 2021·Frontiers in Genetics·Vanessa ChenouardIgnacio Anegon
Jul 2, 2021·Biomacromolecules·Marta Díaz-CaballeroSalvador Ventura
Oct 15, 2021·Biomaterials Science·Angeline PelusCédric Y Montanier

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Methods Mentioned

BETA
chemical modifications
gel filtration
affinity purification
flow cytometry
PCR
genotyping

Software Mentioned

ANANAS
Origami
BioRender

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