Recent advances on separation and characterization of human milk oligosaccharides

Electrophoresis
Veronica MantovaniNicola Volpi

Abstract

Free human milk oligosaccharides (HMOs) are unique due to their highly complex nature and important emerging biological and protective functions during early life such as prebiotic activity, pathogen deflection, and epithelial and immune cell modulation. Moreover, four genetically determined heterogeneous HMO secretory groups are known to be based on their structure and composition. Over the years, several analytical techniques have been applied to characterize and quantitate HMOs, including nuclear magnetic resonance spectroscopy, high-performance liquid chromatography (HPLC), high pH anion-exchange chromatography, off-line and on-line mass spectrometry (MS), and capillary electrophoresis (CE). Even if these techniques have proven to be efficient and simple, most glycans have no significant UV absorption and derivatization with fluorophore groups prior to separation usually results in higher sensitivity and an improved chromatographic/electrophoretic profile. Consequently, the analysis by HPLC/CE of derivatized milk oligosaccharides with different chromophoric active tags has been developed. However, UV or fluorescence detection does not provide specific structural information and this is a key point in particular related to t...Continue Reading

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Citations

Oct 21, 2017·Nutrition Reviews·Stephan ThurlBernd Stahl
Apr 6, 2020·Glycobiology·Sara PorfirioParastoo Azadi
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Feb 16, 2018·Analytica Chimica Acta·Sean Austin, Thierry Bénet
Mar 13, 2018·Chemical Reviews·Grace LuLisa A Holland

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