Recent biotechnological developments in the use of peroxidases
Peroxidases are ubiquitous oxidoreductases that use hydrogen peroxide or alkyl peroxides as oxidants. Advances have recently been made in using them to prepare, under mild and controlled conditions, chiral organic molecules that are valuable for the chemoenzymatic synthesis of a wide range of useful compounds. Horseradish peroxidase can be converted into a peroxygenative enzyme by molecular engineering. Chloroperoxidase, the most versatile peroxidase, behaves like a 'true' monooxygenase in sulfoxidations with molecular oxygen and an external reductant, with substantial increases in enantioselectivity and enzyme stability.
Mechanism of enantioselective oxygenation of sulfides catalyzed by chloroperoxidase and horseradish peroxidase. Spectral studies and characterization of enzyme-substrate complexes
Horseradish peroxidase His-42 --> Ala, His-42 --> Val, and Phe-41 --> Ala mutants. Histidine catalysis and control of substrate access to the heme iron.
Chloroperoxidase as Enantioselective Epoxidation Catalyst: An Efficient Synthesis of (R)-(-)-Mevalonolactone
Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae
Enhancement of hydrogen peroxide stability of a novel Anabaena sp. DyP-type peroxidase by site-directed mutagenesis of methionine residues
Investigating the structural and functional effects of mutating Asn glycosylation sites of horseradish peroxidase to Asp
Low-frequency dynamics of Caldariomyces fumago chloroperoxidase probed by femtosecond coherence spectroscopy
Sulfoxidation mechanism of vanadium bromoperoxidase from Ascophyllum nodosum. Evidence for direct oxygen transfer catalysis
The coprophilous mushroom Coprinus radians secretes a haloperoxidase that catalyzes aromatic peroxygenation
Alkene cleavage catalysed by heme and nonheme enzymes: reaction mechanisms and biocatalytic applications
One of the possible mechanisms for the inhibition effect of Tb(III) on peroxidase activity in horseradish (Armoracia rusticana) treated with Tb(III)
Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides
Stabilization of chloroperoxidase by polyethylene glycols in aqueous media: kinetic studies and synthetic applications
Kinetic and stability studies on the chloroperoxidase complexes in presence of tert-butyl hydroperoxide
Chlorinations catalyzed by chloroperoxidase occur via diffusible intermediate(s) and the reaction components play multiple roles in the overall process
An updated view on horseradish peroxidases: recombinant production and biotechnological applications
How modification of accessible lysines to phenylalanine modulates the structural and functional properties of horseradish peroxidase: a simulation study
Dendrimer-encapsulated Pt nanoparticles with peroxidase-mimetic activity as biocatalytic labels for sensitive colorimetric analyses
Scalable High-Performance Production of Recombinant Horseradish Peroxidase from E. coli Inclusion Bodies.
Cascade enzymes within self-assembled hybrid nanogel mimicked neutrophil lysosomes for singlet oxygen elevated cancer therapy
Cluster screening: an effective approach for probing the substrate space of uncharacterized cytochrome P450s
Bioinformatics in Biomedicine
Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.