Recent developments in the application of P450 based biocatalysts

Current Opinion in Chemical Biology
Yifeng WeiHuimin Zhao

Abstract

Cytochrome P450 monooxygenases (P450s) catalyze regioselective and stereoselective oxidative modifications of a wide variety of substrates, and are involved in the biosynthesis of many natural products. Despite the complex requirements of the P450 reaction system and its intransigence to recombinant expression, the promise of harnessing P450 reactivity for the industrial-scale production of specialty chemicals has led to much effort invested in P450 engineering. Here we review recent developments (between 2015 and 2017) in the application of P450s and their engineered variants as biocatalysts. We describe strategies for the reconstitution of P450 activity in heterologous microbial hosts, and the expanding repertoire of non-natural reactions catalyzed by engineered P450s.

Citations

Jan 31, 2019·Chemistry : a European Journal·Jiakun XuZhiqi Cong
Jan 4, 2019·Chembiochem : a European Journal of Chemical Biology·Guangde JiangYousong Ding
Dec 8, 2019·The Journal of Biological Chemistry·Zhong LiWei Zhang
Apr 4, 2020·Chembiochem : a European Journal of Chemical Biology·Chengchang ZhangYijun Chen
May 6, 2020·Biotechnology and Bioengineering·Moritz B BuerglerBernd Nidetzky
Feb 25, 2019·Engineering in Life Sciences·Chen ShenBoyang Yu
May 4, 2018·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A V KuzikovV V Shumyantseva
Apr 3, 2019·ChemSusChem·Roger A Sheldon, Dean Brady
Dec 10, 2019·Chemical Society Reviews·Ulrich MarkelUlrich Schwaneberg
Aug 21, 2019·International Journal of Biological Macromolecules·Alberto CiaramellaGiovanna Di Nardo
Nov 11, 2019·Emerging Topics in Life Sciences·David Tew
Feb 6, 2021·Biotechnology Advances·Carmen ArandaAna Gutiérrez
Feb 19, 2021·Nature Communications·Peter M HeinemannBernhard Hauer
Jan 27, 2021·Chemistry : a European Journal·Christian SchnepelNorbert Sewald
Aug 20, 2019·Archives of Biochemistry and Biophysics·Mallory KatoLionel Cheruzel
Sep 4, 2020·Biotechnology Advances·Yuyao RenYongjin J Zhou
Sep 27, 2020·Journal of Inorganic Biochemistry·Celine Eidenschenk, Lionel Cheruzel
Mar 7, 2021·Journal of Industrial Microbiology & Biotechnology·Yuxuan YeTodd K Hyster
Sep 16, 2018·Biosensors & Bioelectronics·Victoria V ShumyantsevaAlexander I Archakov
Mar 14, 2019·ACS Synthetic Biology·Ran Zuo, Yousong Ding
Aug 20, 2020·Journal of the American Chemical Society·Savvas LoukaSam P de Visser
Sep 21, 2021·Chembiochem : a European Journal of Chemical Biology·Libo Zhang, Qian Wang
Jul 5, 2021·Biotechnology Advances·Lunjie WuYi-Lei Zhao

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