Recent Developments of the Synthetic Biology Toolkit for Clostridium

Frontiers in Microbiology
Rochelle C JosephNicholas R Sandoval

Abstract

The Clostridium genus is a large, diverse group consisting of Gram-positive, spore-forming, obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well as several industrially relevant species with the ability to produce chemical commodities, particularly biofuels, from renewable biomass. Additionally, other species are studied for their potential use as therapeutics. Although metabolic engineering and synthetic biology have been instrumental in improving product tolerance, titer, yields, and feed stock consumption capabilities in several organisms, low transformation efficiencies and lack of synthetic biology tools and genetic parts make metabolic engineering within the Clostridium genus difficult. Progress has recently been made to overcome challenges associated with engineering various Clostridium spp. For example, developments in CRISPR tools in multiple species and strains allow greater capability to produce edits with greater precision, faster, and with higher efficiencies. In this mini-review, we will highlight these recent advances and compare them to established methods for genetic engineering in Clostridium. In addition, we discuss the current state and development of Clostridium-based...Continue Reading

References

Sep 1, 1989·Plasmid·R C WoolleyM Young
Jan 1, 1996·Applied Biochemistry and Biotechnology·E M Green, G N Bennett
Sep 3, 1999·Applied and Environmental Microbiology·S B TummalaE T Papoutsakis
Aug 30, 2001·Applied and Environmental Microbiology·P W Adcock, C P Saint
Feb 2, 2002·Applied and Environmental Microbiology·Carmela M Gibson, Michael G Caparon
Mar 6, 2003·Journal of Bacteriology·Seshu B TummalaEleftherios T Papoutsakis
Feb 10, 2004·Applied and Environmental Microbiology·Lothar FeustelPeter Dürre
Apr 3, 2004·Nucleic Acids Research·Carlos J ParedesE Terry Papoutsakis
Aug 24, 2006·Molecular Microbiology·Jennifer R O'ConnorJulian I Rood
Mar 14, 2007·Nature Biotechnology·Thomas DrepperKarl-Erich Jaeger
Mar 24, 2007·Science·Rodolphe BarrangouPhilippe Horvath
Jul 31, 2007·Journal of Microbiological Methods·John T HeapNigel P Minton
May 19, 2009·Journal of Microbiological Methods·John T HeapNigel P Minton
Nov 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sang Jun LeeSankar Adhya
Nov 7, 2009·Journal of Microbiological Methods·John T HeapNigel P Minton
Dec 22, 2009·Applied and Environmental Microbiology·Stephen T Cartman, Nigel P Minton
May 11, 2010·Applied and Environmental Microbiology·Clare M CooksleyNigel P Minton
Aug 10, 2010·Applied and Environmental Microbiology·Shital A TripathiNicky C Caiazza
Nov 26, 2010·Applied and Environmental Microbiology·Andrea H HartmanStephen B Melville
Dec 25, 2010·Applied and Environmental Microbiology·Hirofumi NariyaAkinobu Okabe
Jan 26, 2011·MBio·Yili ChenEleftherios T Papoutsakis
Jun 11, 2011·The Journal of Biological Chemistry·Robert P Fagan, Neil F Fairweather
Oct 4, 2011·Applied and Environmental Microbiology·Hirofumi NariyaAkinobu Okabe

❮ Previous
Next ❯

Citations

Aug 15, 2018·Journal of Applied Microbiology·Peter Setlow
Nov 6, 2018·Folia Microbiologica·Bhagwan Rekadwad, Juan M Gonzalez
May 16, 2019·Journal of Bacteriology·Kathleen N McAllister, Joseph A Sorg
Dec 6, 2019·Microbial Biotechnology·Joyshree GangulyRichard van Kranenburg
May 10, 2020·Applied and Environmental Microbiology·François WaselsNicolas Lopes Ferreira
Aug 15, 2020·World Journal of Microbiology & Biotechnology·Teng BaoShang-Tian Yang
Sep 8, 2020·Journal of Industrial Microbiology & Biotechnology·Charlotte Anne VeesStefan Pflügl
Jul 23, 2020·Frontiers in Bioengineering and Biotechnology·Wentao DingShuobo Shi
Aug 14, 2020·MSystems·Catherine M MageeneyKelly P Williams
May 7, 2020·Biotechnology and Bioengineering·Sofia FerreiraIsabel Rocha
Nov 18, 2018·Applied Microbiology and Biotechnology·Amel LatifiMyriam Brugna
May 5, 2020·Frontiers in Bioengineering and Biotechnology·Seong Woo KwonYu-Sin Jang
Aug 28, 2020·Journal of Industrial Microbiology & Biotechnology·SongI HanGeorge N Bennett
Jan 9, 2021·Frontiers in Genetics·Ellen M E SykesAyush Kumar
Aug 23, 2019·Biotechnology Advances·Luísa Czamanski NoraRafael Silva-Rocha
Apr 22, 2020·Current Opinion in Biotechnology·Michael Köpke, Séan D Simpson
Nov 5, 2020·Biotechnology and Applied Biochemistry·Yexin DaiXianhua Liu
Feb 18, 2021·FEMS Microbiology Reviews·Barbara BourgadeM Ahsanul Islam
May 6, 2019·Biotechnology Advances·Peng-Fei XiaMatthew Wook Chang
Jul 29, 2018·Metabolic Engineering·Kamil CharubinEleftherios T Papoutsakis
Oct 20, 2019·Trends in Microbiology·Minyeong YooPhilippe Soucaille
Apr 27, 2021·Applied Microbiology and Biotechnology·Mamou DialloAna M López-Contreras
Jun 8, 2019·Microbiology Spectrum·S A KuehneD Lyras
Jun 25, 2021·Frontiers in Bioengineering and Biotechnology·Qingzhuo WangSheng Yang

❮ Previous
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Methods Mentioned

BETA
gene knockdown
RNA-seq

Software Mentioned

RNAMotif
WEBLOGO
TransTerm
ClosTron

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