Recent H3N2 influenza virus clinical isolates rapidly acquire hemagglutinin or neuraminidase mutations when propagated for antigenic analyses

Journal of Virology
Benjamin S ChambersScott E Hensley

Abstract

Prior to serological testing, influenza viruses are typically propagated in eggs or cell culture. Recent human H3N2 strains bind to cells with low avidity. Here, we isolated nine primary H3N2 viral isolates from respiratory secretions of children. Upon propagation in vitro, five of these isolates acquired hemagglutinin or neuraminidase mutations that increased virus binding to cell surfaces. These mutations can potentially confound serological assays commonly used to identify antigenically novel influenza viruses.

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Related Concepts

Respiratory Secretion
Cell Culture Techniques
Influenza
Neuraminidase
Virus Attachment
Hemagglutinin
Orthomyxoviridae
In Vitro [Publication Type]

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