Jul 14, 2016

Recent Outbreaks of Shigellosis in California Caused by Two Distinct Populations of Shigella sonnei With Increased Virulence or Fluoroquinolone Resistance

BioRxiv : the Preprint Server for Biology
Varvara K KozyrevaVishnu Chaturvedi

Abstract

Shigella sonnei has caused unusually large outbreaks of shigellosis in California in 2014 - 2015. Preliminary data indicated the involvement of two distinct yet related bacterial populations, one from San Diego and San Joaquin (SD/SJ) and one from the San Francisco (SF) Bay area. Whole genome sequencing of sixty-eight outbreak and archival isolates of S. sonnei was performed to investigate the microbiological factors related to these outbreaks. Both SD/SJ and SF populations, as well as almost all of the archival S. sonnei isolates belonged to sequence type 152 (ST152). Genome-wide SNP analysis clustered the majority of California (CA) isolates to an earlier described global Lineage III, which has persisted in CA since 1986. Isolates in the SD/SJ population had a novel Shiga-toxin (STX)-encoding lambdoid bacteriophage, most closely related to that found in an Escherichia coli O104:H4 strain responsible for a large outbreak. However, the STX genes ( stx1a and stx1b ) from this novel phage had sequences most similar to the phages from S. flexneri and S. dysenteriae . The isolates in the SF population yielded evidence of fluoroquinolone resistance acquired via the accumulation of point mutations in gyrA and parC genes. Thus, the CA...Continue Reading

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Mentioned in this Paper

Genome-Wide Association Study
CCL18 gene
Dysentery, Shigella Dysenteriae
Shiga Toxins
STX1A gene
Fluoroquinolones
Genes
Toxin
Bacteriophages
2-butenal

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