Recent progress in structural biology: lessons from our research history

Microscopy
Ryo NittaEriko Nitta

Abstract

The recent 'resolution revolution' in structural analyses of cryo-electron microscopy (cryo-EM) has drastically changed the research strategy for structural biology. In addition to X-ray crystallography and nuclear magnetic resonance spectroscopy, cryo-EM has achieved the structural analysis of biological molecules at near-atomic resolution, resulting in the Nobel Prize in Chemistry 2017. The effect of this revolution has spread within the biology and medical science fields affecting everything from basic research to pharmaceutical development by visualizing atomic structure. As we have used cryo-EM as well as X-ray crystallography since 2000 to elucidate the molecular mechanisms of the fundamental phenomena in the cell, here we review our research history and summarize our findings. In the first half of the review, we describe the structural mechanisms of microtubule-based motility of molecular motor kinesin by using a joint cryo-EM and X-ray crystallography method. In the latter half, we summarize our structural studies on transcriptional regulation by X-ray crystallography of in vitro reconstitution of a multi-protein complex.

References

Oct 1, 1990·Biochemical Society Transactions·R HendersonK H Downing
Oct 25, 1990·Nucleic Acids Research·C Aslanidis, P J de Jong
Jan 1, 1981·Annual Review of Biophysics and Bioengineering·J DubochetC A Walter
Oct 17, 1998·Journal of Structural Biology·F J Sigworth
Feb 12, 1999·Science·F J AsturiasR D Kornberg
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y Okada, N Hirokawa
May 25, 2001·Nature·M KikkawaN Hirokawa
Aug 3, 2004·Science·Ryo NittaNobutaka Hirokawa
Nov 30, 2004·Nature Biotechnology·Imre BergerTimothy J Richmond
Jan 13, 2006·Nature Chemical Biology·Yuichi TaniguchiToshio Yanagida
Aug 4, 2006·Molecular Cell·Yuichiro TakagiRoger D Kornberg
Nov 23, 2006·Nature Methods·Daniel J FitzgeraldImre Berger
Feb 13, 2007·Nature Methods·Mamie Z Li, Stephen J Elledge
Sep 23, 2008·Nature Structural & Molecular Biology·Ryo NittaNobutaka Hirokawa
Apr 17, 2009·Structure·Gang CaiFrancisco J Asturias
Sep 24, 2009·Nature Reviews. Molecular Cell Biology·Nobutaka HirokawaShinsuke Niwa
Nov 26, 2009·Nature Reviews. Molecular Cell Biology·Nobutaka HirokawaYasushi Okada
Dec 6, 1947·Nature·M F PERUTZ, O WEISZ
Jul 5, 2011·Nature·Tsuyoshi ImasakiYuichiro Takagi
Sep 22, 2011·Journal of Structural Biology·Anna-Clare MilazzoClinton S Potter
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Philip J J RobinsonRoger D Kornberg
Mar 29, 2014·Science·Werner Kühlbrandt
Dec 4, 2014·Nature·Julian von der EckenStefan Raunser
Feb 19, 2015·Nature Reviews. Molecular Cell Biology·Benjamin L Allen, Dylan J Taatjes
Sep 5, 2015·Nature Methods·Allison Doerr
Feb 27, 2016·Science·Julia MahamidWolfgang Baumeister

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Citations

Jan 5, 2021·Frontiers in Molecular Biosciences·Anushka HalderSaraswathi Vishveshwara

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