PMID: 6167183Feb 27, 1981Paper

Recent studies on the involvement of retinyl phosphate as a carrier of mannose in biological membranes

Annals of the New York Academy of Sciences
Y ShidojiL M De Luca

Abstract

Rat liver microsomes synthesized [14C]mannosylretinylphosphate and dolichyl [14C]mannosylphosphate from guanosinedisphosphate [14C]mannose, retinylphosphate and dolichylphosphate. Two distinct enzyme activities were shown to be responsible for the biosynthesis of the two mannolipids. A higher affinity mannosyl transferase (EA I), responsible for dolichylmannosylphosphate synthesis, displayed a Km for GDP-mannose of 1.7 microM; while a lower affinity enzyme (EA II), responsible for mannosylretinylphosphate synthesis, displayed a Km for GDP-mannose of 12.5 microM. These Km values were unaffected by the addition of either dolichylphosphate for EA II, or retinylphosphate for EA I. The same Km values were found before and after solubilization of the enzyme activity with 1% Triton X-100. Differential solubilization of EA I and EA II was demonstrated, utilizing different concentrations of Triton X-100. Triple-labeled mannosylretinylphosphate was prepared from [3H]retinylphosphate, retinyl[32P]phosphate and GDP-[14C]mannose from incubations containing rat liver microsomes. This compound was shown to donate [14C]mannose to endogenous acceptors of rat liver microsomes.

References

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Citations

Jun 11, 1986·Biochimica Et Biophysica Acta·C K Ramachandran, G Melnykovych
Jul 15, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·K A LatifP B Rama Rao
Sep 6, 1985·Biochimica Et Biophysica Acta·J Frot-CoutazR Got
Apr 19, 1984·The New England Journal of Medicine·D S Goodman
Nov 15, 1985·European Journal of Biochemistry·J W Jensen, J S Schutzbach
Jul 1, 1986·Experimental Eye Research·L Degenstein, A A Moscona
Mar 1, 1984·Bioscience Reports·R Datema, R T Schwarz

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