Receptor and betagamma binding sites in the alpha subunit of the retinal G protein transducin

Science
R OnrustH R Bourne

Abstract

Transmembrane receptors for hormones, neurotransmitters, light, and odorants mediate their cellular effects by activating heterotrimeric guanine nucleotide-binding proteins (G proteins). Crystal structures have revealed contact surfaces between G protein subunits, but not the surfaces or molecular mechanism through which Galphabetagamma responds to activation by transmembrane receptors. Such a surface was identified from the results of testing 100 mutant alpha subunits of the retinal G protein transducin for their ability to interact with rhodopsin. Sites at which alanine substitutions impaired this interaction mapped to two distinct Galpha surfaces: a betagamma-binding surface and a putative receptor-interacting surface. On the basis of these results a mechanism for receptor-catalyzed exchange of guanosine diphosphate for guanosine triphosphate is proposed.

References

Jan 1, 1991·Annual Review of Biochemistry·Y KaziroT Satoh
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·L Birnbaumer
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P C Sternweis, A G Gilman
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·L A QuilliamS Campbell-Burk
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T C ThomasE J Neer
Dec 29, 1995·The Journal of Biological Chemistry·S Osawa, E R Weiss
Jan 25, 1996·Nature·D G LambrightP B Sigler
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·O LichtargeF E Cohen

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Citations

Sep 6, 2002·Journal of Peptide Science : an Official Publication of the European Peptide Society·Anna Maria DursiPaolo Rovero
Sep 7, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maren ClausRalf Paschke
Feb 7, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Frederick J Ehlert
Feb 2, 2010·Journal of Molecular Modeling·Andrea Strasser, Hans-Joachim Wittmann
Jan 27, 2004·Biochemical and Biophysical Research Communications·Yasuhiro TsunodaChung Owyang
Mar 24, 2004·Journal of Molecular Biology·I MihalekO Lichtarge
Aug 12, 1999·European Journal of Pharmacology·E C HulmeC A Curtis
Jan 28, 2003·Journal of Molecular Biology·Hui YaoOlivier Lichtarge
Jul 24, 2003·Journal of Molecular Biology·I MihalekO Lichtarge
Jan 15, 1999·Pharmacology & Therapeutics·J Wess
Apr 1, 1997·Current Opinion in Cell Biology·H R Bourne
Aug 1, 1997·Current Opinion in Biotechnology·A BohmP B Sigler
Feb 13, 2002·Current Opinion in Structural Biology·Olivier Lichtarge, Mathew E Sowa
Jan 9, 2003·Trends in Biochemical Sciences·Jacqueline Cherfils, Marc Chabre
Oct 6, 2001·Progress in Retinal and Eye Research·I M Pepe
Sep 6, 2008·Biochemistry·Anita M PreiningerHeidi E Hamm
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Aug 1, 1997·Nature Structural Biology·Y WangP B Sigler
Aug 8, 2006·Nature Structural & Molecular Biology·William M OldhamHeidi E Hamm
Sep 12, 2013·Bioinformatics·Angela D WilkinsOlivier Lichtarge
May 12, 2000·Critical Care Medicine·R A Forse
Jan 19, 2005·The FEBS Journal·Aalt D J van DijkAlexandre M J J Bonvin
Feb 19, 2009·Photochemistry and Photobiology·Najmoutin G AbdulaevKevin D Ridge
Jul 29, 2010·Molecular Pharmacology·Marykate CrouthamelPhilip B Wedegaertner
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Oct 27, 2010·PLoS Computational Biology·Brenda R S TempleAlan M Jones
Dec 3, 2013·Nature Structural & Molecular Biology·Nathan S AlexanderJens Meiler
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·O G KisselevK P Hofmann
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·O G KisselevG R Marshall

❮ Previous
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