Receptor binding and biological effect of insulin in human adipocytes

Diabetologia
O AndersenS Gammeltoft

Abstract

The binding of 125I-labelled insulin to human adipocytes was studied at 37 degrees C. The precipitability of the 125I-labelled insulin preparation (0.03 nmol/l) in trichloroacetic acid and the concentration of biologically active insulin (7.5 nmol/l) remained constant in buffer incubated with human adipocytes (100 microliter cells/ml suspension) for 30--60 minutes at 37 degrees C, whereas more than half of the insulin was inactivated by rat fat cells under the same conditions. A constant level of binding of 125I-labelled insulin (0.03 nmol/l) to human adipocytes was obtained after 45 minutes. The apparent dissociation constant of receptor binding was about 0.2 nmol/l as compared to about 2 nmol/l for rat adipocytes. Conversion of [UP14C]glucose to lipids was stimulated half-maximally by about 0.05 nmol/l of insulin (similar to rat adipocytes). Thus, half-maximal stimulation of human adipocytes was obtained with a recptor occupancy of about 20--30 per cent.

References

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Citations

Sep 1, 1983·Diabetologia·M A KhokherP Dandona
Aug 1, 1979·Diabetologia·H Beck-Nielsen, O Pedersen
Oct 1, 1988·The Journal of Clinical Investigation·C R Kahn, M F White
Mar 9, 2010·PloS One·Mark R SommerfeldNorbert Tennagels
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Related Concepts

Fat Pad
Metazoa
Anhydrous Dextrose
Insulin B Chain
Lipids
Insulin Receptor beta Subunit
Rats, Laboratory
In Vitro [Publication Type]

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