Receptor Characterization and Functional Activity of Pyrokinins on the Hindgut in the Adult Mosquito, Aedes aegypti

Frontiers in Physiology
Aryan Lajevardi, Jean-Paul V Paluzzi

Abstract

Pyrokinins are structurally related insect neuropeptides, characterized by their myotropic, pheromonotropic and melanotropic roles in some insects, but their function is unclear in blood-feeding arthropods. In the present study, we functionally characterized the pyrokinin-1 and pyrokinin-2 receptors (PK1-R and PK2-R, respectively), in the yellow fever mosquito, Aedes aegypti, using a heterologous cell system to characterize their selective and dose-responsive activation by members of two distinct pyrokinin subfamilies. We also assessed transcript-level expression of these receptors in adult organs and found the highest level of PK1-R transcript in the posterior hindgut (rectum) while PK2-R expression was enriched in the anterior hindgut (ileum) as well as in reproductive organs, suggesting these to be prominent target sites for their peptidergic ligands. In support of this, PRXa-like immunoreactivity (where X = V or L) was localized to innervation along the hindgut. Indeed, we identified a myoinhibitory role for a PK2 on the ileum where PK2-R transcript was enriched. However, although we found that PK1 did not influence myoactivity or Na+ transport in isolated recta, the PRXa-like immunolocalization terminating in close associa...Continue Reading

References

Nov 30, 1992·Biochemical and Biophysical Research Communications·T KawanoA Suzuki
Jan 1, 1986·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·G M HolmanR J Nachman
Sep 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·L SchoofsA De Loof
Mar 28, 1998·The Journal of Biological Chemistry·M R BrownA O Lea
Dec 24, 1998·British Journal of Pharmacology·A D MichelP P Humphrey
Oct 21, 1999·Insect Biochemistry and Molecular Biology·M A Riehle, M R Brown
Dec 6, 2001·Cell and Tissue Research·M Y ChoiR A Jurenka
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Laura KeanJulian A T Dow
Aug 13, 2002·The Journal of Biological Chemistry·Geert BaggermanLiliane Schoofs
Sep 3, 2002·Mechanisms of Development·Xiaojuan MengChristophe Roos
Aug 11, 2004·Developmental Biology·Peter VerleyenLiliane Schoofs
Oct 23, 2004·The Journal of Experimental Biology·Valerie P PollockShireen-A Davies
Aug 2, 2005·Biochemical and Biophysical Research Communications·Giuseppe CazzamaliCornelis J P Grimmelikhuijzen
Apr 8, 2006·Biochemical and Biophysical Research Communications·Takashi HommaToshinobu Yaginuma
Sep 5, 2006·Cellular and Molecular Life Sciences : CMLS·R Predel, C Wegener
May 19, 2007·Science·Vishvanath NeneDavid W Severson
Aug 22, 2007·Biochemical and Biophysical Research Communications·Stine S OlsenFrank Hauser
Jan 22, 2008·The Journal of Experimental Biology·Victoria A Te BruggeIan Orchard
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mark R BrownMichael R Strand
Sep 9, 2008·General and Comparative Endocrinology·N Audsley, R J Weaver
Jan 20, 2009·The Journal of Experimental Biology·Geoffrey M Coast
Feb 19, 2010·Journal of Proteome Research·Reinhard PredelRonald J Nachman
May 8, 2010·Progress in Neurobiology·Dick R Nässel, Asa M E Winther
May 1, 2012·General and Comparative Endocrinology·Paweł MarciniakGrzegorz Rosiński

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Citations

Oct 15, 2020·ELife·Nora G PetersonDonald T Fox

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Datasets Mentioned

BETA
EAT35008.1
AY539746
KC155994.1
AAEL005444

Methods Mentioned

BETA
ELISA
Assay
PCR
Scanning Electrode Technique
electron microscopy

Software Mentioned

Powerlab
GraphPad Prism
LabChart Pro
GraphPad
ASET
Automated Scanning Electrode Technique ( ASET )
PowerUP

Related Concepts