Receptor-independent infection of murine coronavirus: analysis by spinoculation.

Journal of Virology
Rie WatanabeF Taguchi

Abstract

A highly neurovirulent murine coronavirus JHMV (wild-type [wt] JHMV) is known to spread from cells infected via the murine coronavirus mouse hepatitis virus receptor (MHVR) to cells without MHVR (MHVR-independent infection), whereas a mutant virus isolated from wt JHMV, srr7, spread only in an MHVR-dependent fashion. These observations were obtained by the overlay of JHMV-infected cells onto receptor-negative cells that are otherwise resistant to wt JHMV infection. MHVR-independent infection is hypothetically thought to be attributed to a naturally occurring fusion activation of the wt JHMV S protein, which did not occur in the case of srr7. Attachment of S protein on cells without MHVR during the S-protein activation process seems to be a key condition. Thus, in the present study, we tried to see whether wt JHMV virions that are attached on MHVR-negative cells are able to infect those cells. In order to make virions attach to the cell surface without MHVR, we have used spinoculation, namely, the centrifugation of cells together with inoculated virus at 3,000 rpm for 2 h. This procedure forces viruses to attach to the cell surface, as revealed by quantitative estimation of attached virions by real-time PCR and also facilitated ...Continue Reading

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Citations

Jan 7, 2010·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hanae TakatsukiRihito Watanabe
Mar 12, 2010·Uirusu·Fumihiro Taguchi, Shutoku Matsuyama
Jan 24, 2014·Japanese Journal of Infectious Diseases·Masatoshi KakizakiRihito Watanabe
Jan 26, 2011·Microbes and Infection·Takasuke FukuharaYoshiharu Matsuura
Dec 16, 2011·Journal of Medical Virology·Kazuya ShiratoShutoku Matsuyama
Apr 29, 2016·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Rihito WatanabeAkira Togayachi
May 17, 2012·Viruses·Taylor Heald-Sargent, Tom Gallagher
May 8, 2021·Mayo Clinic Proceedings·John C O'HoroAndrew D Badley

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