Receptor-induced internalization of selective peptidic mu and delta opioid ligands.

The Journal of Biological Chemistry
G GaudriaultJ P Vincent

Abstract

The binding and internalization of radioiodinated and fluorescent mu and delta opioid peptides in mammalian cells were quantitatively studied by biochemical techniques and directly visualized by confocal microscopy. The labeled peptides were prepared by inserting either a 125I-Bolton-Hunter group or a fluorescent probe into the C-terminal part of 5-aminopentylamide derivatives of deltorphin-I and [Lys7]dermorphin. The purified derivatives kept most of their specificity and selectivity toward delta and mu opioid receptors, respectively. Biochemical and confocal microscopy data showed that both mu and delta opioid peptides were internalized in mammalian cells transfected with the corresponding opioid receptor according to a receptor-mediated mechanism. The internalization process was time- and temperature-dependent and was completely blocked by the endocytosis inhibitor phenylarsine oxyde. Internalization of both delta and mu ligands occurred from a single large cap at one pole of the cell, indicating that polymerization of ligand-receptor complexes preceeded internalization. Finally, green and red fluorescent analogues of deltorphin-I and [Lys7]dermorphin, respectively, were found to internalize through partly distinct endocytic...Continue Reading

References

Dec 18, 1991·European Journal of Biochemistry·E Smythe, G Warren
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W LutzR Kumar
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V ErspamerG Kreil
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A GoldsteinS R Newman
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E HazumP Cuatrecasas
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J Halpern, P M Hinkle
Mar 1, 1981·International Journal of Peptide and Protein Research·P C MontecucchiV Erspamer
Aug 1, 1995·Current Opinion in Cell Biology·C Lamaze, S L Schmid
Apr 15, 1994·Journal of Neuroscience Research·M E AboodQ Tao
Nov 8, 1994·Biochemistry·M R TotaC D Strader
Nov 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S L Schmid, H Damke
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·R AshworthP M Hinkle
Mar 3, 1995·The Journal of Biological Chemistry·E F GradyN W Bunnett
Mar 1, 1995·The Journal of Cell Biology·B F RoettgerL J Miller
Sep 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R R Neubig
Mar 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K N Klotz, A J Jesaitis
Nov 1, 1995·Trends in Neurosciences·R A Nixon, A M Cataldo
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·D A EdwardsR Langer

❮ Previous
Next ❯

Citations

Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J ZhangM G Caron
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·P Y LawH H Loh
Mar 21, 2006·Clinical and Experimental Hypertension : CHE·Crystal Hill-Pryor, Joseph C Dunbar
May 12, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·P W SchillerN N Chung
Nov 30, 2004·Life Sciences·Eva V VargaHenry I Yamamura
Dec 17, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jian LuZaijie Jim Wang
Jan 28, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A BeaudetJ P Vincent
Nov 26, 1999·Journal of Cellular Biochemistry·S I BriandG Guillemette
Aug 3, 2002·Nature Reviews. Molecular Cell Biology·Alexander Sorkin, Mark Von Zastrow
Oct 19, 2005·British Journal of Pharmacology·Yvan DumontRémi Quirion
Dec 13, 2005·Journal of Neurophysiology·Heather N WenkChristopher N Honda
Jan 10, 2006·Brain : a Journal of Neurology·Marylou V SolbrigGeorge F Koob
Dec 4, 2009·International Journal of Peptide Research and Therapeutics·Jane V AldrichThomas F Murray
Mar 13, 2014·British Journal of Pharmacology·X ZhangS Li
Mar 29, 2014·British Journal of Pharmacology·Louis GendronWendy Walwyn
May 23, 2014·The Journal of Biological Chemistry·Achla GuptaLakshmi A Devi
Jul 6, 2014·British Journal of Pharmacology·A GuptaL A Devi
Feb 7, 2001·The Journal of Biological Chemistry·P I Tsao, M von Zastrow
Jun 20, 1998·The Journal of Biological Chemistry·R D PolakiewiczM J Comb
Nov 28, 2000·Molecular Pharmacology·S ArttamangkulD K Grandy
Jan 21, 2001·The Journal of Biological Chemistry·G InnamoratiM Birnbaumer
Jul 1, 2020·ChemPlusChem·Antonios Drakopoulos, Michael Decker
Aug 12, 1999·European Journal of Pharmacology·R SchulzK Schulz
Aug 26, 1998·Drug and Alcohol Dependence·H AkilS Watson
Apr 4, 1998·Brain Research. Molecular Brain Research·E A AfifyH H Loh
Mar 18, 1999·Progress in Neurobiology·L H LazarusS Salvadori
Nov 13, 1998·Journal of Pharmaceutical Sciences·R D EgletonT P Davis

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