Receptor-interacting protein 140 is a repressor of the androgen receptor activity

Molecular Endocrinology
Sophie CarascossaStéphan Jalaguier

Abstract

The androgen receptor (AR) is a ligand-activated transcription factor that controls growth and survival of prostate cancer cells. In the present study, we investigated the regulation of AR activity by the receptor-interacting protein 140 (RIP140). We first showed that RIP140 could be coimmunoprecipitated with the receptor when coexpressed in 293T cells. This interaction appeared physiologically relevant because chromatin immunoprecipitation assays revealed that, under R1881 treatment, RIP140 could be recruited to the prostate-specific antigen encoding gene in LNCaP cells. In vitro glutathione S-transferase pull-down assays provided evidence that the carboxy-terminal domain of AR could interact with different regions of RIP140. By means of fluorescent proteins, we demonstrated that ligand-activated AR was not only able to translocate to the nucleus but also to relocate RIP140 from very structured nuclear foci to a diffuse pattern. Overexpression of RIP140 strongly repressed AR-dependent transactivation by preferentially targeting the ligand binding domain-dependent activity. Moreover, disruption of RIP140 expression induced AR overactivation, thus revealing RIP140 as a strong AR repressor. We analyzed its mechanism of transrepre...Continue Reading

References

Mar 31, 1995·The Journal of Biological Chemistry·G JensterA O Brinkmann
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Nov 1, 1996·Molecular and Cellular Biology·F L'HorsetM G Parker
Jun 17, 1998·Molecular Endocrinology·E TreuterJ A Gustafsson
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·R G Roeder
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·A O BrinkmannJ Trapman
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·M J McPhaul
Dec 28, 1999·Molecular and Cellular Endocrinology·S ThenotV Cavailles
Aug 10, 2000·The Journal of Biological Chemistry·M SharmaZ Sun
Sep 28, 2000·The Journal of Biological Chemistry·L N WeiM Farooqui
Mar 5, 2002·Molecular Endocrinology·Elin HolterEckardt Treuter
Apr 12, 2002·Endocrine Reviews·Cynthia A Heinlein, Chawnshang Chang
Jun 29, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Edward P Gelmann
Sep 19, 2002·Endocrine-related Cancer·Z CuligA Hobisch
Sep 21, 2002·Biochimica Et Biophysica Acta·Kristen E Neely, Jerry L Workman
May 30, 2003·Molecular and Cellular Biology·Hiroshi TazawaJohanna Zilliacus
Jan 23, 2004·The Journal of Biological Chemistry·Mark ChristianMalcolm G Parker
Apr 3, 2004·Nucleic Acids Research·Audrey CastetVincent Cavaillès
Oct 16, 2004·The Prostate·Liang WangChawnshang Chang
Dec 2, 2004·Molecular and Cellular Biology·Ding-Yen LinHsiu-Ming Shih
Feb 3, 2005·Molecular and Cellular Biology·Borja BelandiaMalcolm G Parker

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Citations

Apr 17, 2008·The Proceedings of the Nutrition Society·James Thorne, Moray J Campbell
Apr 23, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aurélie DocquierVincent Cavaillès
May 26, 2012·PloS One·Aurélie DocquierVincent Cavaillès
Jan 21, 2014·Molecular and Cellular Endocrinology·Lavinia VijaMarc Lombès
Nov 21, 2007·FEBS Letters·Roger WhiteMalcolm G Parker
Feb 24, 2007·Molecular and Cellular Endocrinology·Allison H MaffeyJuan Ausió
Nov 14, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dexiang ZhangHoubao Liu
Jan 5, 2011·Biochimica Et Biophysica Acta·Meritxell RosellMalcolm G Parker
Sep 16, 2014·Frontiers in Immunology·Duncan HowieStephen Cobbold
Nov 8, 2006·Nuclear Receptor Signaling·Patrick AugereauVincent Cavaillès
Apr 28, 2007·Nuclear Receptor Signaling·Alexander GriekspoorRob Michalides
Jun 10, 2010·American Journal of Physiology. Endocrinology and Metabolism·Asmaà FritahMalcolm G Parker
Jul 15, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel Hormaechea-AgullaRaúl M Luque
Jul 26, 2013·Molecular Endocrinology·Aurélie DocquierVincent Cavaillès
Apr 18, 2017·Oncogene·S JalaguierV Cavaillès
Sep 18, 2014·Cancer Research·Ken-Ichi TakayamaSatoshi Inoue
Jul 4, 2006·Trends in Endocrinology and Metabolism : TEM·Mark ChristianMalcolm G Parker

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