PMID: 9433481Jan 20, 1998Paper

Receptor-mediated and protein kinase-dependent growth enhancement of primary human fibroblasts by platelet activating factor

Molecular Carcinogenesis
S A Bennett, H C Birnboim

Abstract

Chronic inflammation is a recognized risk factor for human cancer, but the causal mechanisms are poorly understood. We previously demonstrated that platelet activating factor (PAF) can induce alterations in the in vitro growth properties of primary rat fibroblasts. In the study reported here, exposure of primary human skin fibroblasts to PAF for 1 h in serum-free medium was shown to cause sustained proliferation over 50 d in medium containing low serum and anchorage-independent growth in soft agarose. Both properties could be inhibited by pretreatment with a PAF receptor antagonist, CV3988 (10 microM); a tyrosine-kinase inhibitor, genistein (1 microgram/mL); or a protein kinase C (PKC) inhibitor, staurosporine (50 nM) but not with a cyclooxygenase inhibitor, indomethacin (200 nM-20 microM). PAF had no effect on doubling time, saturation density, or cell viability under normal monolayer growth conditions in complete medium. Treatment with lyso-PAF, an inactive metabolite of PAF, had no effect in either of the assays. Control and PAF-induced cell proliferation in low-serum medium was inhibited by PAF receptor antagonists present during the extended growth period. The presence of PAF receptor mRNA in human skin fibroblasts was dem...Continue Reading

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Citations

May 9, 2012·Plastic Surgery International·Patrick S Murphy, Gregory R D Evans
Jun 27, 2006·Plastic and Reconstructive Surgery·George BroughtonChristopher E Attinger
Aug 28, 2007·Cancer Metastasis Reviews·Vladislava Melnikova, Menashe Bar-Eli
Sep 3, 1998·Molecular Carcinogenesis·F Rougier, Y Denizot

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