PMID: 6169759Sep 1, 1981Paper

Receptor-mediated endocytosis in cultured fibroblasts: cryptic coated pits and the formation of receptosomes

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M C WillinghamI Pastan

Abstract

Concentrative receptor-mediated endocytosis of many specific ligands by cultured fibroblasts occurs through the coated pit-receptosome pathway. The formation of receptosomes was studied using two impermeant electron-dense labels for the cell surface, ruthenium red and concanavalin A-horseradish peroxidase. These studies show that at 4 degrees C, virtually all coated structures near the plasma membrane are in communication with the cell surface, and are not isolated coated vesicles. On warming cells to 37 degrees C for only 1 minute, a major portion of these structures become cryptic, that is, not labeled by these surface markers. However, on cooling cells immediately back to 4 degrees C, virtually all of these structures are again in communication with the surface. Many images showed that membrane of these cryptic pits to be continuous with the cell surface when caught in the appropriate plane of section; often there was a very narrow entrance that excluded extracellular label. At 37 degrees C, receptosomes could be occasionally seen forming as an invagination of membrane adjacent to the coated region. Mechanisms by which receptosomes may form and other evidence demonstrating the failure of coated pits to pinch off to form isol...Continue Reading

Citations

Jan 1, 1984·Molecular and Cellular Biochemistry·P J Davies, M P Murtaugh
Jan 1, 1983·Anatomy and Embryology·I M MacLean, E J Sanders
Jan 1, 1984·Pharmacology & Therapeutics·K C Zoon, H Arnheiter
Aug 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·P V NestorF Gannon
Apr 27, 2000·Trends in Biochemical Sciences·P BarnettE H Hettema
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J Y FanL Orci
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R B DicksonI Pastan
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M C Willingham, I Pastan
Dec 1, 1990·The Journal of Cell Biology·S L Schmid, L L Carter
Jul 1, 1982·The Journal of Cell Biology·M C Willingham, I H Pastan
Nov 1, 1983·The Journal of Cell Biology·C Hertel, M Staehelin
Oct 1, 1984·The Journal of Cell Biology·N K GonatasJ O Gonatas
Jan 1, 1982·Journal of Interferon Research·L M Pfeffer, I Tamm
Oct 30, 2009·BMC Genomics·Susanne A ThomsonGerald A Leblanc
Feb 2, 2008·BMC Structural Biology·Lucas BleicherIgor Polikarpov
Jun 19, 2014·International Journal of Nanomedicine·Tianshu Li, Shinji Takeoka
Oct 15, 1981·Biochemical and Biophysical Research Communications·R SchlegelI Pastan
May 14, 1984·Brain Research·J E Smith, D O Smith
Nov 1, 2005·Developmental Cell·David Perrais, Christien J Merrifield
Dec 1, 1984·The Anatomical Record·J WatanabeM Oka
Nov 1, 1983·The Journal of Experimental Zoology·F W Bazer, R M Roberts
Apr 1, 1981·Experimental Cell Research·M C WillinghamI H Pastan
Sep 1, 1984·The American Journal of Anatomy·L Hermo, C Morales
Dec 1, 1985·Experimental Cell Research·K L GoldenthalM C Willingham
Jun 1, 1984·The Anatomical Record·C MoralesY Clermont
Jul 1, 1985·The American Journal of Anatomy·C MoralesL Hermo
May 1, 1983·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C J Steer, R D Klausner
Jan 1, 1982·Annals of the New York Academy of Sciences·R B DicksonI H Pastan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.