Receptor-mediated endocytosis of decorin: involvement of leucine-rich repeat structures

Archives of Biochemistry and Biophysics
H HausserH Kresse

Abstract

Decorin, a small dermatan sulfate proteoglycan, is characterized by a core protein with central leucine-rich repeat structures and a single glycosaminoglycan chain. It is catabolized by receptor-mediated uptake and subsequent intralysosomal degradation. In the present study, the localization of the receptor binding site(s) along the core protein was investigated. Various recombinant decorin fragments were consistently able to inhibit the endocytosis of wild-type decorin. The most potent inhibitory peptides were those which encompassed the central Leu125-Val230 region, i.e., the fifth to eighth leucine-rich repeat, or at least a part of it. The peptide Leu125-Val230 bound directly to the 51-kDa endocytosis receptor, and Fab fragments of antibodies against this peptide inhibited the endocytosis of decorin in a dose-dependent manner. Decorin constructs expressed in human 293 cells and comprising the full-length coding region or lacking sequences N- and/or C-terminally of the Leu125-Val230 region were all endocytosed with similar clearance rates. These data suggest that the N- and C-terminal domains of the core protein are not required for endocytosis. The receptor binding site is rather represented by contiguous leucine-rich repea...Continue Reading

References

Jun 1, 1987·The Journal of Cell Biology·G SchmidtH Kresse
Jun 1, 1987·Collagen and Related Research·K G Vogel, J A Trotter
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M SantraR V Iozzo
Sep 1, 1995·The Journal of Biological Chemistry·L SvenssonA Oldberg
Apr 14, 1995·The Journal of Biological Chemistry·E SchönherrH Kresse
Feb 10, 1995·The Journal of Biological Chemistry·E SchönherrH Kresse
May 15, 1993·Experientia·H KresseE Schönherr
Aug 9, 1996·The Journal of Biological Chemistry·P RamamurthyD J McQuillan
Dec 13, 1996·The Journal of Biological Chemistry·I T WeberR V Iozzo
Feb 10, 1997·The Journal of Cell Biology·K G DanielsonR V Iozzo

❮ Previous
Next ❯

Citations

Oct 13, 2012·European Journal of Cell Biology·David Denis Sofeu FeugaingManuela Viola
Dec 9, 1998·Kidney International·L SchaeferH Kresse
Jun 13, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Rebeca DroguettEnrique Brandan
Apr 16, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Enrique Brandan, Jaime Gutierrez
Feb 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D DallD O'Reilly
Jan 3, 2012·Journal of Burn Care & Research : Official Publication of the American Burn Association·Dariush HonardoustEdward E Tredget
Oct 22, 2002·FEBS Letters·Ellen Tufvesson, Gunilla Westergren-Thorsson
Aug 29, 2006·The Journal of Biological Chemistry·Enrique BrandanMaría-Paz Marzolo
May 9, 2007·The Journal of Biological Chemistry·Claudio Cabello-Verrugio, Enrique Brandan
Feb 21, 2008·The Journal of Biological Chemistry·Sumit BhattacharyyaJoanne K Tobacman
Apr 7, 2005·The Journal of Biological Chemistry·Vinay M BhideChristopher A McCulloch
Jun 8, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·A D WinterC J Handley
Nov 23, 2000·European Journal of Cell Biology·E SchönherrH Kresse

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.