PMID: 8611608Jan 12, 1996Paper

Receptor-mediated endocytosis of poly(acrylic acid)-conjugated liposomes by macrophages

Biochimica Et Biophysica Acta
M FujiwaraR H Grubbs

Abstract

The uptake characteristics of negatively-charged liposomes made by conjugation of poly(acrylic acid) (PAA) were studied with respect to cultured RAW macrophages. The PAA-conjugated liposomes were internalized and digested in an acidic compartment at a much faster rate than the unmodified phosphatidylcholine (PC) liposomes. After incubation for 18 h, an over 5-fold increase in the uptake of PC liposomes was obtained by PAA conjugation. Subsequently, part of the aqueous phase of the internalized liposomes was exocytosed. Recognition of PAA by the macrophages seems to be responsible for the enhanced uptake of PAA-conjugated liposomes. Cross-competition experiments showed that PAA-conjugated liposomes inhibited the uptake of acetylated-low density lipoprotein (acetyl-LDL) by the macrophages and vice versa. The uptake of PAA-conjugated liposomes was also inhibited by dextran sulfate and maleylated-bovine serum albumin (maleyl-BSA), which are also known to bind to scavenger receptors. Poly(C) and BSA, which are not ligands for the scavenger receptor, competed poorly with the uptake of PAA-conjugated liposomes. Enhanced uptake of PAA-conjugated liposomes by CHO cells with low scavenger receptor expression was not observed. Unexpectedl...Continue Reading

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Citations

Apr 7, 2004·Advanced Drug Delivery Reviews·Marie-Andrée Yessine, Jean-Christophe Leroux
Sep 1, 2007·Bioconjugate Chemistry·Elena V BatrakovaHoward E Gendelman
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·J A KampsG L Scherphof
Apr 17, 2010·Nanomedicine·Anna M BrynskikhElena V Batrakova
May 3, 2008·Journal of Pharmaceutical Sciences·Giulia BonacucinaGiovanni F Palmieri
Oct 15, 2011·Advanced Drug Delivery Reviews·Arnaud E FelberJean-Christophe Leroux
Jun 25, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eiji YubaKenji Kono
Jan 1, 2007·Progress in Polymer Science·A V Kabanov, H E Gendelman
Feb 9, 2010·Journal of Drug Targeting·Linglin FengWeiyue Lu
Feb 1, 2011·Expert Opinion on Drug Delivery·Keitaro SouWilliam T Phillips
May 20, 2015·ACS Biomaterials Science & Engineering·Yixue SuJian Yang
Aug 26, 2016·Journal of Liposome Research·Nikolaos NazirisCostas Demetzos
Feb 26, 2011·Expert Opinion on Drug Delivery·Elena V BatrakovaAlexander V Kabanov
Dec 31, 2015·PloS One·Andrea L Martinez-SkinnerHoward E Gendelman
Jul 9, 2008·Journal of Microencapsulation·Shailja TiwariSuresh P Vyas
Oct 1, 2005·Journal of Liposome Research·Xuedong YanJan A A M Kamps
Apr 11, 2018·Oxidative Medicine and Cellular Longevity·Nicholas E BuglakEdward S M Bahnson
Dec 13, 2019·Future Science OA·Manuela Giraldo-VillegasMauricio Rojas-López
Aug 28, 2020·Expert Opinion on Drug Delivery·Claire H MastersonJohn G Laffey

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