Receptor-mediated modulation of recombinant neuronal class E calcium channels

FEBS Letters
G MehrkeT Schneider

Abstract

The modulation of a cloned neuronal calcium channel was studied in a human embryonic kidney cell line (HEK293). The HEK293 cells were stably transfected with the alpha1Ed cDNA, containing the pore forming subunit of a neuronal class E calcium channel. Inward currents of 25 +/- 1.9 pA/pF (n = 79) were measured with the cloned alpha1Ed-subunit. The application of the peptide hormone somatostatin, carbachol, ATP or adenosine reduced the amplitude of Ca2+ and Ba2+ inward currents and exhibited a slowing of inactivation. This inhibitory effect by somatostatin was significantly impaired after pre-incubating the transfected cell line with pertussis toxin (PTX). Internal perfusion of the cells with the G-protein-inactivating agent GDP-beta-S or with the permanently activating agent GTP-gamma-S also attenuated the somatostatin effect. The inhibition indicates that modulation of the alpha1Ed-mediated Ca2+ current involves pertussis toxin-sensitive G-proteins. The block of Ca2+ and Ba2+ inward currents by somatostatin is also found in cells expressing a truncated alpha1Ed-subunit which lacks a 129-bp fragment in the C-terminus. This fragment corresponds to the major structural difference between two native human alpha1E splice variants. A...Continue Reading

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Citations

Apr 23, 2003·European Journal of Pharmacology·Atticus H HainsworthAndrew D Randall
Aug 2, 2002·Biochimica Et Biophysica Acta·Jennifer W MitchellPhilip M Best
Sep 30, 1998·The European Journal of Neuroscience·A PereverzevT Schneider
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·L G WuB Sakmann
Mar 28, 2008·BMC Physiology·Vicenta Salvador-RecatalàRobert M Greenberg
Mar 18, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Andreas KriegerToni Schneider
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Kayalvizhi RadhakrishnanToni Schneider
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Kayalvizhi RadhakrishnanToni Schneider
Mar 3, 1998·The Journal of Physiology·A C Dolphin
Jun 25, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Siarhei A SiapichMatthias Lüke
Oct 16, 2012·Biochimica Et Biophysica Acta·Gerald W Zamponi, Kevin P M Currie
Aug 4, 1998·Current Opinion in Neurobiology·G W Zamponi, T P Snutch
Jan 21, 2015·Biochimica Et Biophysica Acta·Irina DrobinskayaToni Schneider
Jul 29, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H GrabschT Schneider
May 23, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M WeiergräberT Schneider
Nov 28, 2013·Pharmaceuticals·Toni SchneiderJürgen Hescheler
Nov 30, 2006·Pharmacological Reviews·H William Tedford, Gerald W Zamponi
Jun 20, 2020·The Journal of General Physiology·Felix NeumaierToni Schneider
Oct 3, 1999·Physiological Reviews·F Lehmann-Horn, K Jurkat-Rott

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