Receptor-mediated transfection of murine and ovine mammary glands in vivo.

The Journal of Biological Chemistry
A S SobolevL K Ernst

Abstract

Transfection of HC-11 murine epithelial mammary cells as well as murine and sheep mammary glands were carried out using insulin-containing constructs that deliver DNA by receptor-mediated endocytosis to receptor-expressing cells. In vivo transfection of mammary gland tissue with the luciferase gene was carried out by introducing the DNA constructs into the mammary ducts of both mice and sheep. The successful transfection of ewe mammary glands was demonstrated by the detection of luciferase activity in mammary gland biopsy material up to a month after a single administration of the construct. To test whether products of expression of transfected genes could be secreted into the milk in this system, the N-terminal secretory signal sequences of bovine beta-lactoglobulin or the entire coding sequence of human alpha-lactalbumin were fused to the N terminus of the luciferase gene. After transfection with the modified luciferases, both murine and sheep milk could be shown to contain luciferase activity, whereas mice, which had been transfected with the nonmodified luciferase gene, did not secrete any activity in the milk. This approach demonstrates for the first time the possibility of gene transfer in vivo into mammary gland epitheli...Continue Reading

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Citations

Sep 2, 1999·Molecular Reproduction and Development·M M IvanovaL K Ernst
Apr 27, 2000·Progress in Biophysics and Molecular Biology·A S SobolevA A Rosenkranz
Jan 9, 2001·Trends in Biotechnology·J Gariépy, K Kawamura
Aug 18, 2000·Immunology and Cell Biology·A A RosenkranzA S Sobolev
Jul 12, 2002·The Journal of Gene Medicine·Markus DüchlerMathias Müller
Aug 15, 2003·World Journal of Gastroenterology : WJG·Xiao-Hong OuYu-Guo Zhong
Jul 31, 2004·World Journal of Gastroenterology : WJG·Xiao-Hong OuYu-Guo Zhong
Oct 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xinhai WangDavid W Pascual
Jun 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·A A YaroslavovF M Menger
Jan 1, 2005·Biotechnology and Bioengineering·M Laird ForrestDaniel W Pack

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