Receptor microscopic autoradiography for the study of percutaneous absorption, in vivo skin penetration, and cellular-intercellular deposition

Journal of Pharmacological and Toxicological Methods
Naohiko HayakawaW E Stumpf

Abstract

Microscopic autoradiography with cellular resolution and preservation of in vivo conditions is potentially the method of choice to gain detailed information about sites of deposition and retention in the epidermis and of penetration to the dermis after topical application of drugs. We tested this using (3)H-Maxacalcitol. Dorsal skin of adult rats was treated in vivo with ointment containing 1 or 40 microg/kg body weight of the vitamin D analogue (3)H-Maxacalcitol for periods of 0.5, 2, 8, 24, 48, or 168 h. Samples of skin exposed to the ointment and control samples remote from the treatment site were excised and freeze-mounted, and 4-microm frozen sections were exposed to nuclear emulsion. Two penetration routes to the dermis could be distinguished: one via epidermal cell layers and the other via hair follicles. Highest uptake and retention of radiolabeled steroid was observed in stratum corneum and in intercellular spaces of stratum granulosum. By contrast, cell boundaries and intercellular spaces in the stratum spinosum and basale contained low levels of radioactivity. Keratinocytes in these layers showed high concentration in the cytoplasm at early time intervals, when surrounding radioactivity levels were high, but high nuc...Continue Reading

References

May 1, 1992·Journal of Cellular Biochemistry·K Kragballe
Jan 1, 1995·Archives of Dermatological Research·W E StumpfK Kumaki
Sep 1, 1995·Journal of Cellular Biochemistry·X Q TianM F Holick

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Citations

Apr 19, 2011·European Journal of Drug Metabolism and Pharmacokinetics·Walter E Stumpf
Nov 6, 2007·Experimental Dermatology·Walter E StumpfHans-Juergen Bidmon
Jan 23, 2009·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Cormac Flynn, Brendan A O McCormack
May 13, 2014·International Journal for Numerical Methods in Biomedical Engineering·J Weickenmeier, M Jabareen
Oct 14, 2019··Louise BastholmAmin Feizpour

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