Receptor potential characteristics during direct stereocilia stimulation of isolated outer hair cells from the guinea-pig

Acta Physiologica Scandinavica
Y N Shvarev, B Canlon

Abstract

The receptor potential as a function of stimulus amplitude and frequency was studied with the patch-clamp technique in isolated outer hair cells (OHCs) with a length ranging from 30 to 87 microm during direct mechanical stimulation of the stereocilia. The amplitude and frequency of the stimulation were varied from 125 nm to 2 microm and from 100 Hz to 2.5 kHz, respectively. The mean resting membrane potential before stimulation was -64.25 +/- 1.4 mV (mean +/- SE, n = 26). Irrespective of the frequency used, stereocilia stimulation produced a combination of AC and DC responses, and both components showed saturation with increasing stimulation. Frequency responses appeared to be a function of intensity and resembled a low-pass filter with a time constant ranging from 0.2 to 2.0 ms. With increasing stereocilia stimulation, the relative contribution of high frequencies to the AC component decreased, suggesting a decrease of the corner frequency. The saturated amplitude of the AC component for low-frequency stimulation (100 Hz) was proportional to cell length and increased with a mean rate of 0.014 mV microm(-1). A relationship between the DC response of the receptor potential and the pre-stimulus membrane potential was found. Recor...Continue Reading

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