Receptor-transporting protein 1 short (RTP1S) mediates translocation and activation of odorant receptors by acting through multiple steps.

The Journal of Biological Chemistry
Lifang WuHanyi Zhuang

Abstract

Odorant receptor (OR) proteins are retained in the endoplasmic reticulum when heterologously expressed in cultured cells of non-olfactory origins. RTP1S is an accessory protein to mammalian ORs and facilitates their trafficking to the cell-surface membrane and ligand-induced responses in heterologous cells. The mechanism by which RTP1S promotes the functional expression of ORs remains poorly understood. To obtain a better understanding of the role(s) of RTP1S, we performed a series of structure-function analyses of RTP1S in HEK293T cells. By constructing RTP1S deletion and chimera series and subsequently introducing single-site mutations into the protein, we found the N terminus of RTP1S is important for the endoplasmic reticulum exit of ORs and that a middle region of RTP1S is important for OR trafficking from the Golgi to the membrane. Using sucrose gradient centrifugation, we found that the localization of RTP1S to the lipid raft microdomain is critical to the activation of ORs. Finally, in a protein-protein interaction analysis, we determined that the C terminus of RTP1S may be interacting with ORs. These findings provide new insights into the distinct roles of RTP1S in OR translocation and activation.

References

Jun 23, 1999·Current Opinion in Genetics & Development·P Mombaerts
Nov 4, 2000·The Journal of Biological Chemistry·A A GimelbrantT S McClintock
Sep 15, 2001·Nature·S Firestein
Jan 22, 2002·Nature Neuroscience·Xinmin Zhang, Stuart Firestein
Sep 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chris HagueKenneth P Minneman
Apr 15, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Luiz Eduardo C Von DanneckerBettina Malnic
May 25, 2006·The Journal of Biological Chemistry·Maik BehrensWolfgang Meyerhof
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Luiz Eduardo C Von DanneckerBettina Malnic
Mar 28, 2007·The Journal of Biological Chemistry·Hanyi Zhuang, Hiroaki Matsunami
Sep 18, 2007·Nature·Andreas KellerHiroaki Matsunami
Oct 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Fabien M DécaillotLakshmi A Devi
Mar 6, 2009·Science Signaling·Harumi SaitoJoel D Mainland
Aug 19, 2009·Annals of the New York Academy of Sciences·Hiroaki MatsunamiSandeepa Dey
Oct 15, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erwin IlegemsRobert F Margolskee

❮ Previous
Next ❯

Citations

Apr 16, 2014·The Journal of General Physiology·Zita PeterlinMatthew E Rogers
Feb 11, 2015·Current Opinion in Neurobiology·Yue Jiang, Hiroaki Matsunami
Dec 3, 2014·Analytical Biochemistry·Yosuke FukutaniMasafumi Yohda
Sep 7, 2016·Biosensors & Bioelectronics·Tomasz WasilewskiWojciech Kamysz
Jan 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kentaro IkegamiHiroaki Matsunami
Jan 18, 2018·Chemical Senses·Maik BehrensSimone Weyand
Aug 10, 2019·The Journal of Biological Chemistry·Yosuke FukutaniMasafumi Yohda
Nov 29, 2012·BMB Reports·NaNa Kang, JaeHyung Koo
Jan 20, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthias KotthoffDietmar Krautwurst
Sep 12, 2018·Biosensors & Bioelectronics·John W CaveAlexander N Mitropoulos
Jun 17, 2021·Lab on a Chip·Yusuke HirataShoji Takeuchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.