PMID: 26328272Sep 4, 2015Paper

Receptor tyrosine kinase expression of circulating tumor cells in small cell lung cancer

Oncoscience
Gerhard HamiltonMaximilian Hochmair

Abstract

Small cell lung cancer (SCLC) has a poor prognosis and is found disseminated at first presentation in the majority of cases. The cell biological mechanisms underlying metastasis and drug resistance are not clear. SCLC is characterized by high numbers of circulating tumor cells (CTCs) and we were able to expand several CTC lines ex vivo and to relate chitinase-3-like-1/YKL-40 (CHI3L1) as marker. Availability of expanded SCLC CTC cells allowed for a screening of receptor tyrosine kinases (RTKs) expressed. The metastatic CHI3L1-negative SCLC cell line SCLC26A, established from a pleural effusion was used for comparison. The CTC cell line BHGc10 was found to exhibit increased expression of RYK, AXL, Tie-1, Dtk, ROR1/2, several ephrins (Eph) and FGF/EGF receptors compared to SCLC26A. All of these RTKs have been associated with cell motility, invasion and poor prognosis in diverse cancer entities without knowledge of their association with CTCs. The identification of RYK, AXL and ROR1/2 as pseudokinases, lacking activity, seems to be related to the observed failure of RTK inhibitors in SCLC. These kinases are involved in the noncanonical WNT pathway and their expression in SCLC CTCs represents a cancer stem cell-like phenotype.

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M HovensA F Wilks
Jan 3, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M M Halford, S A Stacker
Nov 17, 2004·Current Pharmaceutical Design·Dana Brantley-SiedersJin Chen
Jul 20, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masuko Katoh, Masaru Katoh
Jun 3, 2009·Cancer Science·Kazuhito MoriokaKoichi Matsuda
Jul 7, 2010·Cell·Mark A Lemmon, Joseph Schlessinger
Aug 31, 2011·The Journal of Cell Biology·Jason D BerndtRandall T Moon
Feb 15, 2012·Cellular Signalling·Hua ZhangGeorgios Giamas
Apr 25, 2012·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Catherine Alix-Panabières
Jul 10, 2012·The Journal of Biological Chemistry·Maria L MachedaSteven A Stacker
Sep 7, 2012·Cold Spring Harbor Perspectives in Medicine·Gavin Thurston, Christopher Daly
Sep 11, 2012·Experimental Cell Research·Jing-Jing Li, Dong Xie
Sep 27, 2012·Nature Genetics·M Catherine Pietanza, Marc Ladanyi
Aug 14, 2013·Stem Cells·Benjamin J Povinelli, Michael J Nemeth
Sep 24, 2013·PloS One·Michael M HalfordSteven A Stacker
Dec 3, 2013·Advances in Experimental Medicine and Biology·Thomas HensingRavi Salgia
Dec 7, 2013·Journal of the National Cancer Institute·David J Stewart
Jan 1, 2014·Nature Reviews. Drug Discovery·Andrew W BoydMartin Lackmann
Feb 20, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gerhard HamiltonTheresia Thalhammer
May 23, 2014·Cancer Chemotherapy and Pharmacology·Monica MitaAnthony W Tolcher
Jul 30, 2014·Thoracic Surgery Clinics·Georgios Stamatis
Aug 22, 2014·British Journal of Cancer·B W DayA W Boyd
Dec 3, 2014·Biochimica Et Biophysica Acta·Christian RolfoPatrick Pauwels
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 9, 2015·Nature Reviews. Cancer·Douglas K GrahamH Shelton Earp
Jan 17, 2015·Cancer Research·Laure CayrefourcqCatherine Alix-Panabières
Apr 9, 2015·Nature Reviews. Clinical Oncology·Naoko TakebeS Percy Ivy

❮ Previous
Next ❯

Citations

Dec 24, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yongan FuYangqiang Wang
Nov 18, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ashwini BalakrishnanStanley R Riddell
Dec 28, 2018·Chemical Reviews·Michael D Paul, Kalina Hristova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.