Receptor tyrosine phosphatase psi is required for Delta/Notch signalling and cyclic gene expression in the presomitic mesoderm

Development
Birgit Aerne, D Ish-Horowicz

Abstract

Segmentation in vertebrate embryos is controlled by a biochemical oscillator ('segmentation clock') intrinsic to the cells in the unsegmented presomitic mesoderm, and is manifested in cyclic transcription of genes involved in establishing somite polarity and boundaries. We show that the receptor protein tyrosine phosphatase psi (RPTPpsi) gene is essential for normal functioning of the somitogenesis clock in zebrafish. We show that reduction of RPTPpsi activity using morpholino antisense oligonucleotides results in severe disruption of the segmental pattern of the embryo, and loss of cyclic gene expression in the presomitic mesoderm. Analysis of cyclic genes in RPTPpsi morphant embryos indicates an important requirement for RPTPpsi in the control of the somitogenesis clock upstream of or in parallel with Delta/Notch signalling. Impairing RPTPpsi activity also interferes with convergent extension during gastrulation. We discuss this dual requirement for RPTPpsi in terms of potential functions in Notch and Wnt signalling.

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Citations

Apr 10, 2008·PloS One·Chetana SachidanandanRandall T Peterson
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Jun 3, 2020·The FEBS Journal·Veronika ReitererHesso Farhan
Jul 16, 2016·Human Molecular Genetics·Zainab AsadChetana Sachidanandan
Jun 28, 2020·Nature Communications·Iain M HayJanet E Deane
Jul 21, 2004·Current Opinion in Genetics & Development·François Giudicelli, Julian Lewis

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