Receptors for hyaluronic acid and poliovirus: a combinatorial role in glioma invasion?

PloS One
Zaynah MaherallyGeoffrey J Pilkington

Abstract

CD44 has long been associated with glioma invasion while, more recently, CD155 has been implicated in playing a similar role. Notably, these two receptors have been shown closely positioned on monocytes. In this study, an up-regulation of CD44 and CD155 was demonstrated in established and early-passage cultures of glioblastoma. Total internal reflected fluorescence (TIRF) microscopy revealed close proximity of CD44 and CD155. CD44 antibody blocking and gene silencing (via siRNA) resulted in greater inhibition of invasion than that for CD155. Combined interference resulted in 86% inhibition of invasion, although in these investigations no obvious evidence of synergy between CD44 and CD155 in curbing invasion was shown. Both siRNA-CD44 and siRNA-CD155 treated cells lacked processes and were rounder, while live cell imaging showed reduced motility rate compared to wild type cells. Adhesion assay demonstrated that wild type cells adhered most efficiently to laminin, whereas siRNA-treated cells (p<0.0001 for both CD44 and CD155 expression) showed decreased adhesion on several ECMs investigated. BrdU assay showed a higher proliferation of siRNA-CD44 and siRNA-CD155 cells, inversely correlated with reduced invasion. Confocal microscop...Continue Reading

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Citations

Apr 17, 2014·BioMed Research International·Chary Marquez BatistaGuilherme Lepski
Nov 26, 2013·Molecular Neurobiology·Eli T SayeghAndrew T Parsa
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Oct 3, 2018·Cellular & Molecular Immunology·Paola Kučan BrlićStipan Jonjić
Jul 25, 2020·Neurological Research·Abraham TsitlakidisNicolas Foroglou

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Methods Mentioned

BETA
surgical resection
flow cytometry
confocal microscopy
GTPase
GTPases
biopsy
transfection
Fluorescence-activated cell sorting
FACS
Assay

Software Mentioned

GraphPad Prism
Volocity Image Analysis
Volocity
AxioVision

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