Receptors involved in carbohydrate binding modulate intestinal epithelial-neutrophil interactions.

The Journal of Biological Chemistry
S P ColganJ L Madara

Abstract

Neutrophil (polymorphonuclear neutrophil) migration across epithelial barriers is a common morphologic feature of many diseases. Previous studies show that PMN-epithelial interactions are dependent on the PMN beta 2-integrin CD11b/18; however, nothing is known about surface carbohydrates and PMN-epithelial interactions. Here we investigate the role of carbohydrates on PMN-epithelial interactions using PMN and cultured monolayers of the intestinal epithelial cell line T84. Addition of the carbohydrates mannose 6-phosphate (Man-6-P) and glucose 6-phosphate (Glu-6-P), but not fructose 1-phosphate (Fru-1-P) inhibited transmigration by > or = 70%. Likewise, more complex carbohydrates, such as fucoidin and the Man-6-P-rich polysaccharide PPME selectively inhibited PMN transepithelial migration. These carbohydrates were found to be inhibitory in the apical-to-basolateral direction as well as the basolateral-to-apical direction, indicating a lack of polarity. This panel of related carbohydrates, however, was not effective in modulating short-term adhesion of PMN to epithelial monolayers, indicating that carbohydrate ligands may modulate different steps in the transmigration cascade. Finally, addition of functionally inhibitory monoclon...Continue Reading

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Citations

Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Parkos
Mar 14, 2000·Veterinary Immunology and Immunopathology·E SmitsA Massart-Leën
Oct 2, 2003·Current Opinion in Cell Biology·Ke Zen, Charles A Parkos
Jan 22, 1998·Journal of Cardiovascular Pharmacology·M OmataT Ohno
Nov 28, 2007·Annual Review of Pathology·Alex C Chin, Charles A Parkos
Nov 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Rachel L ZemansGregory P Downey
Jan 1, 1996·International Journal of Clinical & Laboratory Research·G BertonC A Lowell
Sep 7, 2007·Digestive Diseases and Sciences·A R F BarretoG A C Brito
Apr 17, 2016·The American Journal of Pathology·Charles A Parkos
Oct 9, 2012·Biochemical Pharmacology·Min XuImad Shureiqi
Aug 26, 2016·Immunological Reviews·Jennifer C Brazil, Charles A Parkos
Dec 20, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·L YangC Tian
May 2, 2009·Biochimica Et Biophysica Acta·Rino DonatoBarry C Powell
Jun 18, 2005·Memórias do Instituto Oswaldo Cruz·Titus A ReavesCharles A Parkos
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
Mar 28, 2008·Biochemical Society Transactions·Sarah Garrido-UrbaniBeat A Imhof
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ke ZenCharles A Parkos
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ke ZenYuan Liu
Jun 1, 1997·Arthritis and Rheumatism·C F Mojcik, E M Shevach
Feb 24, 2001·Annals of the New York Academy of Sciences·D L Jaye, C A Parkos
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·S RosseauJ Lohmeyer
Feb 15, 2001·American Journal of Physiology. Cell Physiology·M Si-TaharJ L Madara
Feb 19, 1998·Alimentary Pharmacology & Therapeutics·J L Madara
Oct 27, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer C BrazilNancy A Louis
Feb 6, 2021·Cellular and Molecular Life Sciences : CMLS·Wan-Chi Lin, Michael B Fessler

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