PMID: 6991872Jan 1, 1980Paper

Recessive nonsense-suppression in yeast Saccharomyces cerevisiae: the study of 80 S ribosomes accumulated in suppressor strain under non-permissive conditions

Molecular & General Genetics : MGG
A P SurguchovS G Inge-Vechtomov

Abstract

The shift of recessive suppressor mutant of yeast Saccharomyces cerevisiae from permissive to restrictive conditions is accompanied by polysome decay and accumulation of 80 S ribosomes (Smirnov et al., 1976). In this paper some properties of 80 S ribosomes are studied. It is demonstrated that polysome decay under non-permissive conditions is not the consequence of the impairment of RNA synthesis. More than 70% of 80 S ribosomes accumulated under non-permissive conditions contain bound peptidyl-tRNAs localized in P-ribosomal site. tRNA moiety of bound peptidyl-tRNA is able to accept all 20 natural amino acids after chemical deacylation. Therefore it is not a specific isoacceptor species but rather total tRNA that is bound to ribosomes. The polypeptide residues of these peptidyl-tRNAs are heterogeneous in size. Their molecular weights are comparable with the molecular weights of the completed polypeptides. Some of the 80 S ribosomes accumulated under non-permissive conditions contain poly-A+ RNA. In conclusion, possible mechanism of the impairment of translation under non-permissive conditions in recessive suppressor strain is discussed.

References

Jul 6, 1978·Molecular & General Genetics : MGG·V N SmirnovY V Berestetskaya
Jun 16, 1978·Molecular Biology Reports·A P SurguchovL V Lyzlova
Mar 22, 1976·Molecular & General Genetics : MGG·W L Gerlach
Jan 1, 1975·Molecular & General Genetics : MGG·W L Gerlach
Feb 2, 1976·European Journal of Biochemistry·W H Mager, R J Planta
Mar 14, 1974·Molecular & General Genetics : MGG·V N SmirnovS G Inge-Vechtomov
Mar 27, 1974·Molecular & General Genetics : MGG·N S Petersen, C S McLaughlin
Oct 17, 1972·European Journal of Biochemistry·B A van der ZeijstH P Bloemers
Nov 1, 1968·Journal of Bacteriology·L H Hartwell, C S McLaughlin
Dec 1, 1968·Genetics·D C Hawthorne, R K Mortimer

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Citations

Dec 28, 1999·Annual Review of Cell and Developmental Biology·T R Serio, S L Lindquist
Jan 1, 1982·Molecular & General Genetics : MGG·M D Ter-AvanesyanA P Surguchov
May 1, 1994·Current Genetics·I Stansfield, M F Tuite
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·I L DerkatchS W Liebman
Apr 15, 1981·Journal of Molecular Biology·M MasurekarF Sherman
Apr 15, 1981·Journal of Molecular Biology·J IshiguroF Sherman
Apr 15, 1981·Journal of Molecular Biology·B I OnoF Sherman

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