RECG maintains plastid and mitochondrial genome stability by suppressing extensive recombination between short dispersed repeats

PLoS Genetics
Masaki OdaharaYasuhiko Sekine

Abstract

Maintenance of plastid and mitochondrial genome stability is crucial for photosynthesis and respiration, respectively. Recently, we have reported that RECA1 maintains mitochondrial genome stability by suppressing gross rearrangements induced by aberrant recombination between short dispersed repeats in the moss Physcomitrella patens. In this study, we studied a newly identified P. patens homolog of bacterial RecG helicase, RECG, some of which is localized in both plastid and mitochondrial nucleoids. RECG partially complements recG deficiency in Escherichia coli cells. A knockout (KO) mutation of RECG caused characteristic phenotypes including growth delay and developmental and mitochondrial defects, which are similar to those of the RECA1 KO mutant. The RECG KO cells showed heterogeneity in these phenotypes. Analyses of RECG KO plants showed that mitochondrial genome was destabilized due to a recombination between 8-79 bp repeats and the pattern of the recombination partly differed from that observed in the RECA1 KO mutants. The mitochondrial DNA (mtDNA) instability was greater in severe phenotypic RECG KO cells than that in mild phenotypic ones. This result suggests that mitochondrial genomic instability is responsible for the ...Continue Reading

References

Aug 1, 1992·The Plant Cell·J M Martínez-ZapaterC R Somerville
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·J CourcelleP C Hanawalt
Nov 14, 1997·Immunotechnology : an International Journal of Immunological Engineering·M A ClarkR L Ward
Aug 26, 1999·Trends in Genetics : TIG·H L RaceW Martin
Mar 16, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T NishiyamaM Hasebe
Jul 13, 2000·Journal of Molecular Biology·O EmanuelssonG von Heijne
Nov 7, 2001·The Plant Journal : for Cell and Molecular Biology·Y HiwatashiM Hasebe
Nov 20, 2001·Nucleic Acids Research·S KurtzR Giegerich
Dec 26, 2001·Biochimica Et Biophysica Acta·N Peeters, I Small
Apr 25, 2002·Journal of Experimental Botany·K Van Gestel, J-P Verbelen
Jun 5, 2002·Annual Review of Biochemistry·Shelley L Lusetti, Michael M Cox
Aug 1, 1993·The Plant Cell·J. GuK. J. Newton
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ricardo V AbdelnoorSally A Mackenzie
Nov 18, 2003·Annual Review of Genetics·Justin Courcelle, Philip C Hanawalt
Jan 1, 2004·The Journal of Biological Chemistry·Mara E RobuMichael M Cox
Jan 22, 2004·Nature Reviews. Genetics·Jeremy N TimmisWilliam Martin
Apr 7, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Geoffrey S BriggsRobert G Lloyd
Jun 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhenguo LinHong Ma
Feb 13, 2007·Current Opinion in Plant Biology·Ralph S QuatranoDavid J Cove
May 1, 2007·The Plant Cell·Vikas ShedgeSally A Mackenzie
Nov 27, 2007·The Plant Journal : for Cell and Molecular Biology·Obed W OdomDavid L Herrin
Dec 7, 2007·Nucleic Acids Research·William C Burhans, Martin Weinberger
May 8, 2008·Bioscience, Biotechnology, and Biochemistry·Takayuki InouyeYasuhiko Sekine
Jun 23, 2009·Molecular Microbiology·Christian J RudolphRobert G Lloyd
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexandre MaréchalNormand Brisson
Feb 26, 2010·The New Phytologist·Alexandre Maréchal, Normand Brisson
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Taegun KwonDavid L Herrin

❮ Previous
Next ❯

Citations

Sep 5, 2015·The Plant Journal : for Cell and Molecular Biology·Masaki OdaharaYasuhiko Sekine
Mar 19, 2016·Biochimica Et Biophysica Acta·Joanna MelonekKarin Krupinska
Apr 4, 2016·Current Genetics·Robert G Lloyd, Christian J Rudolph
Feb 6, 2017·FEBS Letters·Benura Azeroglu, David R F Leach
Feb 23, 2017·Annual Review of Plant Biology·José M Gualberto, Kathleen J Newton
May 20, 2016·Scientific Reports·Ilja GaidutšikJuhan Sedman
Jun 4, 2018·Plant & Cell Physiology·Wataru Sakamoto, Tsuneaki Takami
Apr 14, 2017·The Plant Journal : for Cell and Molecular Biology·Masaki OdaharaYasuhiko Sekine
Jan 18, 2020·International Journal of Molecular Sciences·Nicolas ChevignyJosé Manuel Gualberto
May 5, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Karin KrupinskaMichela Zottini

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB646798

Methods Mentioned

BETA
transmission electron microscopy
PCR
phosphotransferase

Software Mentioned

REPuter
TargetP
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.