RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli.

Genetics
Christian J RudolphR G Lloyd

Abstract

Replication of the Escherichia coli chromosome usually initiates at a single origin (oriC) under control of DnaA. Two forks are established and move away in opposite directions. Replication is completed when these meet in a broadly defined terminus area half way around the circular chromosome. RecG appears to consolidate this arrangement by unwinding D-loops and R-loops that PriA might otherwise exploit to initiate replication at other sites. It has been suggested that without RecG such replication generates 3' flaps as the additional forks collide and displace nascent leading strands, providing yet more potential targets for PriA. Here we show that, to stay alive, cells must have either RecG or a 3' single-stranded DNA (ssDNA) exonuclease, which can be exonuclease I, exonuclease VII, or SbcCD. Cells lacking all three nucleases are inviable without RecG. They also need RecA recombinase and a Holliday junction resolvase to survive rapid growth, but SOS induction, although elevated, is not required. Additional requirements for Rep and UvrD are identified and linked with defects in DNA mismatch repair and with the ability to cope with conflicts between replication and transcription, respectively. Eliminating PriA helicase activity...Continue Reading

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Citations

Jul 31, 2013·Nature·Christian J RudolphRobert G Lloyd
Mar 1, 2012·BMC Microbiology·Anna StockumChristian J Rudolph
Nov 1, 2012·BMC Molecular Biology·Michael W KillenAndrew J Pierce
Aug 8, 2014·PLoS Genetics·Julia S P Mawer, David R F Leach
Jan 24, 2015·Progress in Biophysics and Molecular Biology·Piero R Bianco
Apr 4, 2016·Current Genetics·Robert G Lloyd, Christian J Rudolph
Apr 1, 2015·Nucleic Acids Research·Vessela PetrovaMichael M Cox
Jun 12, 2013·Cell Reports·Rémy BétousDavid Cortez
Oct 14, 2016·PloS One·Getachew Tesfaye BeyeneTone Tønjum
Feb 13, 2016·PLoS Genetics·Benura AzerogluDavid R F Leach
Feb 6, 2017·FEBS Letters·Benura Azeroglu, David R F Leach
Dec 14, 2018·Nucleic Acids Research·Sarah L Midgley-SmithChristian J Rudolph
May 1, 2020·Frontiers in Microbiology·Aisha H SyedaChristian J Rudolph
Mar 19, 2019·Molecular Microbiology·Nicklas A HamiltonJustin Courcelle
Oct 27, 2020·Frontiers in Microbiology·Alexia HapeshiNicholas R Waterfield
Jan 1, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Brian M WendelJustin Courcelle

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